Oligonucleotide-Based Therapies, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 2036
Coordonnateurs : Gissberg Olof, Zain Rula, Lundin Karin E.
Part I: Reviews
1. Chemical Development of Therapeutic Oligonucleotides
Karin E. Lundin, Olof Gissberg, C.I. Edvard Smith, and Rula Zain
2. Tissue-Specific Delivery of Oligonucleotides
Xin Xia, Nicolette Pollock, Jiehua Zhou, and John Rossi
Part II: Protocols and Strategies
3. Designing siRNA and Evaluating Its Effect on RNA Targets Using qPCR and Western Blot
Linda Vidarsdottir, Oksana Goroshchuk, Iryna Kolosenko, and Caroline Palm-Apergi
4. Antisense Oligonucleotides for Splice Modulation: Assessing Splice Switching Efficacy
Cristina S.J. Rocha
5. Assessing Oligonucleotide Binding to Double-Strand DNA
Negin Mozafari and Tea Umek
6. Modelling and Simulation of Oligonucleotide Hybrids: Outlining a Strategy
Lennart Nilsson and Alessandra Villa
7. Characterization of Peptide/Oligonucleotide Complexes Using Electron Microscopy, Dynamic Light Scattering, and Protease Resistance Assay
Kärt Padari, Ly Porosk, Piret Arukuusk, and Margus Pooga
8. Click-Shielded and Targeted Lipopolyplexes
Philipp Michael Klein and Ernst Wagner
9. Attachment of Peptides to Oligonucleotides on Solid Support Using Copper(I)-Catalyzed Huisgen 1,3-Dipolar Cycloaddition
Malgorzata Honcharenko, Dmytro Honcharenko, and Roger Stromberg
10. Characterizing Oligonucleotide Uptake in Cultured Cells: A Case Study Using AS1411 Aptamer
Elsa M. Reyes-Reyes and Paula J. Bates
11. Measuring the Action of Oligonucleotide Therapeutics in the Lung at the Cell Type-Specific Level by Tissue Disruption and Cell Sorting (TDCS)
Helen Graves, Steven Evans, Michael Fauler, Manfred Frick, and Sterghios A. Moschos
12. Delivery of Antisense Oligonucleotides Mediated by a Hydrogel System: In Vitro and In Vivo Application in the Context of Spinal Cord Injury
Pedro M.D. Moreno, Teresa Rodrigues, Marília Torrado, Isabel F. Amaral, and Ana P. Pêgo
13. Evaluation of Cell-Penetrating Peptide Delivery of Antisense Oligonucleotides for Therapeutic Efficacy in Spinal Muscular Atrophy
Suzan M. Hammond, Frank Abendroth, Michael J. Gait, and Matthew J.A. Wood
14. Injection and Infusion of Compounds to the Central Nervous System
Margherita Zamboni
15. In Vitro Assessment of the Hepatotoxicity Potential of Therapeutic Oligonucleotides
Sabine Sewing, Tanja Minz, and Franziska Boess
16. Identifying Suitable Target Regions and Analyzing Off-Target Effects
Lykke Pedersen, Peter H. Hagedorn, and Troels Koch
17. RACE-SEQ and Population-Wide Polymorphism Susceptibility Testing for Endonucleolytically Active, RNA-Targeting Therapeutics
Louise Usher, Pantazis I. Theotokis, and Sterghios A. Moschos
18. Studying the Biotransformation of Phosphorothioate-Containing Oligonucleotide Drugs by LC-MS
Christophe Husser, Erich Koller, Andreas Brink, and Simone Schadt
19. ADME: Assessing Pharmacokinetic-Pharmacodynamic Parameters of Oligonucleotides
Rasmus Jansson-Löfmark*, Christine Ahlström, and Peter Gennemark
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Date de parution : 08-2020
Ouvrage de 344 p.
17.8x25.4 cm
Date de parution : 08-2019
Ouvrage de 344 p.
17.8x25.4 cm