Natural Antibodies, Softcover reprint of the original 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1643

This volume looks at the role of natural antibodies in pathogen elimination, cell survival, inflammation, cancer, and autoimmunity. The chapters in this book cover numerous topics, such as isolation of natural antibodies; methods for separating natural antibodies from human plasma, saliva, breast milk, and gastrointestinal fluids; functional properties of natural antibodies such as  anti-tumor cytotoxic activity, and hydrolysis and dissolution of their target antigens; their utility in serological diagnosis of microbial antigens; and the role of natural antibodies in inhibiting viral vectors in the absence of prior exposure to the virus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Natural Antibodies: Methods and Protocols is a valuable resource for researchers and experts who are interested in further studies of natural antibodies.

Preface…
Table of Contents…
Contributing Authors… 

1.      Natural Antibodies: Next Steps towards Translational Investigation
Heinz Kohler 

2.      Isolation of Natural Anti-FceRIa Autoantibodies from Healthy Donors      Monique Vogel, and Michael P. Horn 

1. Isolation of Antibodies from Human Plasma, Saliva, Breast Milk, and Gastrointestinal Fluid
   Christoph Schneider, Marlies Illi, Marius Lötscher, Marc Wehrli, and  Stephan von Gunten 

1. Purification of Natural Antibodies against Tau Protein by Affinity Chromatography
   Michala Kolarova, Lenka Hromadkova, and Jan Ricny 

1. Unbiased RACE-Based Massive Parallel Surveys of Human IgA Antibody Repertoires
   Hanane El Bannoudi, Celine Anquetil, Marc Braunstein, Sergei L. Kosakovsky Pond, and Gregg J. Silverman 

1. Analysis of Signaling Events in B-1a Cells
   Jianxin Huo, Yuhan Huang, Shengli Xu, and Kong-Peng Lam 

Exploring the Role of Microbiota in the Limiting of B1 and MZ B-Cell Numbers by Naturally-Secreted Immunoglobulins
Elodie Mohr and Andreia C. Lino 

1. Assessment of Anti-Tumor Cytotoxic Activity of Naturally Occurring Antibodies in Human Serum or Plasma
   Reinhard Schwartz-Albiez and Othmar Dill 

1. Hydrolysis and Dissolution of Amyloids by Catabodies
   Ville V. Meretoja, Sudhir Paul, and Stephanie A. Planque 

1. Methods for Post-Translational Induction of Polyreactivity of Antibodies
   Maxime Lecerf, Annaelle Jarossay, Srinivas V. Kaveri, Sébastien Lacroix-   Desmazes, and  Jordan D. Dimitrov

1. Characterization of Natural IgM Antibodies Recognizing Oxidation-Specific Epitopes on Circulating Microvesicles
   Florian Puhm and Christoph J. Binder 

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts