Mouse Models of Innate Immunity (2^nd Ed., 2nd ed. 2019) Methods and Protocols Methods in Molecular Biology Series, Vol. 1960

This second edition presents methods and protocols to aid readers in the design and execution of experiments used to define critical elements associated with innate immune system function. New and updated chapters detail protocols on in vitro and ex vivo studies in key cell types associated with innate immunity and with in vivo protocols used to study immune system function in the mouse. Additionally, chapters describe methods to evaluate innate immune function and new protocols associated with autism, cancer, microfluidics platforms, and CRISPR systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols, Second Edition will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune system.

CRISPR/Cas9-Assisted Genome Editing in Murine Embryonic Stem Cells.- Genome Editing in Mouse Embryos with CRISPR/Cas9.- Derivation of Macrophages from Mouse Bone Marrow.- Bone Marrow Derived Dendritic Cells.- Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils.- In Vitro Differentiation of Effector CD4+ T Helper Cell Subsets.- Generation and Culture of Mouse Embryonic Fibroblasts.- Isolation of Tumor-Infiltrating Lymphocytes by Ficoll-Paque Density Gradient Centrifugation.- Depletion and Reconstitution of Macrophages in Mice.- Microfluidic Platform to Quantify Neutrophil Migratory Decision-Making.- A Vector Suite for the Overexpression and Purification of Tagged Outer Membrane, Periplasmic, and Secreted Proteins in E. colz.- Mouse Model of Staphylococcus aureus Skin Infection.- Systemic Listeria monocytogenes Infection as a Model to Study T Helper Cell Immune Responses.- Endotoxin-Induced Uveitis in Rodents.- Using Klebsiella pneumoniae to Model Acute Lung Inflammation in Mice.- Assessment of Survival and Replication of Brucella spp. in Murine Peritoneal Macrophages.- Influenza-Mediated Lung Infection Models.- Adoptive Transfer Colitis.- The Azoxymethane/Il10-/- Model of Colitis Associated Cancer (CAC).- Modeling Autism-Related Disorders in Mice with Maternal Immune Activation (MIA).- Toxoplasma gondii as a Model of In Vivo Host-Parasite Interactions.- Sepsis Induced by Cecal Ligation and Puncture.