Molecular Diagnostics for Melanoma, 2014 Methods and Protocols Methods in Molecular Biology Series, Vol. 1102

​Novel Insights/translational Implication from the Emerging Biology of Melanoma.- Emerging Clinical Issues in Melanoma in the Molecularly Targeted Era.- Integrating Molecular Biomarkers Into Current Clinical Management In Melanoma.- Advances in Adjuvant Therapy: Potential for Prognostic and Predictive Biomarkers.- Immunologic Monitoring of Cancer Vaccine Trials Using the ELISPOT Assay.- Markers for Anti-Cytotoxic T-lymphocyte Antigen 4 (CTLA-4) Therapy in Melanoma.- Marker Utility for Combination Therapy.- Assaying for BRAF V600E in Tissue and Blood in Melanoma.- Selecting Patients for KIT Inhibition in Melanoma.- Detecting Mechanisms of Acquired BRAF inhibitor Resistance in Melanoma.- Current Status of Diagnostic and Prognostic Markers in Melanoma.- Chromosomal Copy Number Analysis in Melanoma Diagnostics.- Construction and Analysis of Multi-Parameter Prognostic Models for Melanoma Outcome.- Immunohistochemical Diagnostic and Prognostic Markers for Melanoma.- Lymphatic Invasion as a Prognostic Biomarker in Primary Cutaneous Melanoma.- Tumor Infiltrating Lymphocytes and Their Significance in Melanoma Prognosis.- Pathological Staging of Melanoma.- Genotyping of Human Leukocyte Antigen (HLA) Ancestral Haplotypes as Prognostic Marker in Cancer Using PCR Analysis.- B7-H Abnormalities in Melanoma and Clinical Relevance.- Melanoma Susceptibility Genes and Risk Assessment.- Clinical, Pathologic, and Imaging Features, and Biological Markers of Uveal Melanoma.- A Prognostic Test to Predict Risk of Metastasis in Uveal Melanoma Based on a 15-Gene Expression Profile.- Molecular Karyotyping for Detection of Prognostic Markers in Fine Needle Aspirate Biopsy Samples of Uveal Melanoma.- ERBB4 Mutation Analysis: Emerging Molecular Target for Melanoma Treatment.- Epigenetic Markers of Prognosis in Melanoma.- Isolation of Melanoma Cell Subpopulations Using Negative Selection.- Circulating Tumor Cells as Prognostic Biomarkers in Cutaneous Melanoma Patients.- Detection ofChondroitin Sulfate Proteoglycan 4 (CSPG4) in Melanoma.- Targeting Damage Associated Molecular Pattern Molecules (DAMPs) and DAMP Receptors in Melanoma.- The Clinical Use of PET/CT in the Evaluation of Melanoma.- Immune System Functional Pathway Analysis Using Single Cell Network Profiling (SCNP): A Novel Tool in Cancer Immunotherapy.- Quantitative and Spatial Image Analysis of Tumor and Draining Lymph Nodes Using Immunohistochemistry and High Resolution Multispectral Imaging to Predict Metastasis.- COLD-PCR Enriches Low-Level Variant DNA Sequences and Increases the Sensitivity of Genetic Testing.- Isolation of Circulating MicroRNAs from Microvesicles found in Human Plasma.- Detection of Circulating Tumor Cells by Photoacustic Flowmetry.- Statistical Design and Evaluation of Biomarker Studies.- Tissue Resources for Clinical Use and Marker Studies in Melanoma.

Seeks to provide both clinicians and scientists with technical information on a wide range of approaches available in the field Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts