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Leishmania, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1971

Langue : Anglais
Couverture de l’ouvrage Leishmania

This volume explores the latest techniques used to study Leishmania and other trypanosomatidae. Chapters in this book cover a wide range of topics such as cultivation of axenic amastigote forms; phylogeny and comparative genomics; and in vitro and in vivo infection models that look at hosts ranging from cell lines to mammalian and arthropode hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Unique and cutting-edge, Leishmania: Methods and Protocols is a valuable guide for both established and novice researchers in the Leishmania field. This book will also be useful to researchers working with other protozoa of the Order Trypanosomatide. 


Preface…
Table of Contents…
Contributing Authors…

1. Host-Free Systems for Differentiation of Axenic Leishmania
Dan Zilberstein and Roni Nitzan Koren

2. Phylogenetic Studies
Katrin Kuhls and Isabel Mauricio

3. A Guide to Next Generation Sequence Analysis of Leishmania Genomes
Hideo Imamura and Jean-Claude Dujardin

4. Quantitative RNA Analysis using RNA Seq
Peter J. Myler, Jacqueline A. McDonald, Pedro Alcolea, and Aakash Sur

5. Ribosome Profiling in Trypanosomatids
Amelie J. Kraus and Raúl O. Cosentino

6. Cosmid Library Construction and Functional Cloning
Joachim Clos and Dorotheo Zander-Dinse

7. Cos-Seq, A High Throughput Gain of Function Screen for Drug Resistance Studies in Leishmania
Jade-Eva Potvin, Philippe Leprohon, Elodie Gazanion, Mansi Sharma, Christopher Fernandez-Prada, and Marc Ouellette

8. Gene Replacement by Homologous Recombination
Henner Zirpel and Joachim Clos

9. LeishGEdit: A Method for Rapid Gene Knockout and Tagging using CRISPR-Cas9
Tom Beneke and Eva Gluenz

10. DiCre-Based Inducible Disruption of Leishmania Genes
Samuel M. Duncan, Elmarie Myburgh, Eliza Alves-Ferreira, and Jeremy C. Mottram

11. DiCre-Based Inducible Gene Expression
Jeziel D. Damasceno, Luiz R. O. Tosi, Renato E. R. S. Santos, and Jeremy C. Mottram

12. Generation of Bone Marrow-Derived Macrophages for In Vitro Infection Experiments
Eugenia Bifeld

13. Quantification of Intracellular Leishmania spp. Using Real-Time Quantitative PCR (qPCR)
Eugenia Bifeld

14. In Vitro Infections of Macrophage-Like Cell Lines with Leishmania infantum for Drug Screening
Nuno Santarém, Joana Tavares, and Anabela Cordeiro-da-Silva

15. Quantification of Parasite Loads by Automated Microscopic Image Analysis
Carolina Borsoi Moraes and Laura Maria Alcântara

16. Quantification of Leishmania Parasites in Murine Models of Visceral Infection
Joana Tavares, Nuno Santarém, and Anabela Cordeiro-da-Silva

17. Syrian Hamster as an Advanced Experimental Model for Visceral Leishmaniasis
María Dolores Jiménez-Antón, Montserrat Grau, Ana Isabel Olías-Molero, and José Mª Alunda

18. Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation versus Chronicity of Disease
Christian Bogdan, Andrea Debus, Heidi Sebald, Bapli Rai, Johanna Schäfer, Stephanie Obermeyer, and Ulrike Schleicher

19. Establishment, Maintenance of Phlemotomus spp. In the Laboratory, and Infection with Leishmania spp.
Ifhem Chelbi and Elyes Zhioua

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Date de parution :

Ouvrage de 370 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

179,34 €

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