Introduction to Experimental Biophysics (Set) Textbook and Lab Manual

This set presents wet lab methods for courses in biophysics or molecular biology. It provides a thorough description of modern experimental and analytical techniques used in biological and biophysical research. The author gives practical, step-by-step guidance on instrumentation and experimental design and includes recipes for common solutions and media, lists of important reagents, and a glossary of biological terms.

The manual offers a flexible course plan that permits completion of the labs in either a full term or intensive summer course. Tested in a pedagogical setting, the experiments follow a logical progression beginning with a DNA construct. The book starts with the basics of molecular cloning: amplifying and purifying plasmid, plasmid mapping, and using restriction enzymes. Later experiments deal with more advanced, emerging techniques, such as the synthesis and characterization of quantum dots and gold nanoparticles, protein crystallization, and spectroscopic techniques.

Prologue: Glossary and Introduction to the Wet Lab. Introduction and Background. Dna and Rna Methods. Protein Expression Methods. Protein Characterization Methods. Introduction to Light Microscopy. Advanced Microscopy Techniques. Expression Systems. Quantitative Tissue Culture Techniques. Surface Functionalization. Electrophysiology. Advanced Cell-Labeling Techniques. Nanoparticle Methods. Advanced Fluorescence Methods. Spectroscopy Tools and Techniques. Appendix 1: Quantitative Data Analysis. Appendix 2: Useful Lab Data.