Immunometabolism, 1st ed. 2020 Methods and Protocols Methods in Molecular Biology Series, Vol. 2184

1. Single-Cell Transcriptomics of Immune Cells: Cell Isolation and cDNA Library Generation for scRNA-Seq

            Janilyn Arsenio

2. Monitoring Lactate Dynamics in Individual Macrophages with a Genetically-Encoded Probe

            Felipe Baeza-Lehnert, Carlos A. Flores, Anita Guequen, and L. Felipe Barros

3. The Conjugation of Antibodies for the Simultaneous Detection of Surface Proteins and Transcriptome Analysis at a Single-Cell Level

            Iivari Kleino, Eliisa Kekäläinen, and Tapio Lönnberg

4. Cellular Fatty Acid Analysis in Macrophage Using Stable Isotope Labeling

            Kevin J. Williams and Steven J. Bensinger

5. Proteomic Network Analysis of Polarized Macrophages

            Jayanta K. Chakrabarty, Abu Hena Mostafa Kamal, A.D.A. Shahinuzzaman, and Saiful M. Chowdhury

6. The Purification and Characterization of Exosomes from Macrophages

            Eran Fridman, Lana Ginini, Ziv Gil, and Neta Milman

7. The Isolation and Characterization of T Lymphocyte Exosomes Using Mass Spectrometry

            Inbar Azoulay-Alfaguter and Adam Mor

8. Analysis of Immune-Tumor Cell Interactions Using a 3D Co-Culture Model

            Tanya N. Augustine

9. Studying Adipocyte and Immune Cell Crosstalk Using a Co-Culture System

            Jennifer M. Monk, Danyelle M. Liddle, Amber L. Hutchinson, and Lindsay E. Robinson

10. A Gene Expression Analysis of M1 and M2 Polarized Macrophages

            Nour Eissa, Hayam Hussein, and Jean-Eric Ghia

11. Simultaneous, Quantitative Characterization of Protein ADP-Ribosylation and Protein Phosphorylation in Macrophages

            Casey M. Daniels, Arthur Nuccio, Pauline R. Kaplan, and Aleksandra Nita-Lazar

12. The Analysis of Mycobacterium tuberculosis-Induced Bioenergetic Changes in Infected Macrophages Using an Extracellular Flux Analyzer

            Bridgette M. Cumming, Vineel P. Reddy, and Adrie J.C. Steyn

13. Analyzing the Metabolic Phenotype of Bone Marrow-Derived Dendritic Cells by Assessing Their Oxygen Consumption and Extracellular Acidification

            Hsi-Ju Wei, John J. Letterio, and Tej K. Pareek

14. The Evaluation of Mitochondrial Membrane Potential Using Fluorescent Dyes or a Membrane-Permeable Cation (TPP⁺) Electrode in Isolated Mitochondria and Intact Cells

            João S. Teodoro, Ivo F. Machado, Ana C. Castela, Anabela P. Rolo and Carlos M. Palmeira

15. The Multiparametric Analysis of Mitochondrial Dynamics in T Cells from Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs)

            Jesse J.R. Masson, Matias Ostrowski, Gabriel Duette, Man kit Sam Lee, Andrew J. Murphy, Suzanne M. Crowe, and Clovis S. Palmer

16. The Measurement of Whole Body Glucose Homeostasis in Mice

            Yang Xin Zi Xu, Sudharsana R. Ande, and Suresh Mishra

17. Immunometabolism and Its Potential to Improve the Current Limitations of Immunotherapy

            Andrew D. Sheppard and Joanne Lysaght

18. Sex Differences in Immunometabolism: An Unexplored Area

            Suresh Mishra, Geetika Bassi, and Yang Xin Zi Xu

19. Isolation and Preparation of Bone Marrow-Derived Immune Cells for Metabolic Analysis

            Nnamdi Ikeogu, Chidalu A. Edechi, Gloria Akaluka, Aida Feiz-Barazandeh, and Jude E. Uzonna

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts