Immunocytochemistry in Diagnostic Cytology

Cytology refers to a branch of pathology that deals with making diagnoses of diseases and conditions through the examination of tissue samples from the body (MedicineNet.com).

Immunocytochemistry is a laboratory method that uses antibodies to check for certain antigens (markers) in a sample of cells. The antibodies are usually linked to an enzyme or a fluorescent dye. After the antibodies bind to the antigen in the cell sample, the enzyme or dye is activated, and the antigen can then be seen under a microscope. Immunocytochemistry is used to help diagnose diseases, such as cancer. It may also be used to help tell the difference between different types of cancer (National Cancer Institute).

This book is a comprehensive guide to the techniques and application of immunocytochemistry in cytology.

Divided into two parts, the first section discusses basic principles and preparation, techniques and quality control, and automated immune staining.

Section Two covers diagnostic applications of immunocytochemistry for many different types of tumour.

The text is highly illustrated with microphotographs, tables and boxes to assist learning and interpretation of findings for accurate diagnosis.

Key points

• Comprehensive guide to techniques and application of immunocytochemistry in cytology
• Provides thorough understanding of basic principles and methods
• Covers diagnostic applications for many different types of tumour
• Highly illustrated with microphotographs, tables and boxes

Part I: Practical aspects of immunocytochemistry

• Chapter 1 Basic principles of immunocytochemistry
• Chapter 2 Visualization of antigen antibody reaction
• Chapter 3 Basic preparation for immune-staining
• Chapter 4 Practical technique, quality control and troubleshooting in immunocytochemistry
• Chapter 5 Automated immune-staining

Part II: Diagnostic applications of immunocytochemistry

• Chapter 6 Essential antibodies for routine application in laboratory
• Chapter 7 Immunocytochemistry of effusion
• Chapter 8 Immunocytochemistry in undifferentiated malignancy and tumors of the unknown primary site
• Chapter 9 Immunocytochemistry in head, neck, salivary gland and thyroid lesions
• Chapter 10 Immunocytochemistry in lung tumours
• Chapter 11 Immunocytochemistry of lymph node
• Chapter 12 Immunocytochemistry of breast tumours
• Chapter 13 Immunocytochemistry of gastrointestinal tract tumours
• Chapter 14 Immunocytochemistry of liver and bile duct tumours
• Chapter 15 Immunocytochemistry of pancreatic tumours
• Chapter 16 immunocytochemistry of kidney and urinary tract tumours
• Chapter 17 Immunocytochemistry of ovarian tumours
• Chapter 18 Immunocytochemistry of soft tissue tumours

Pranab Dey MD MIAC FRCPath
Professor, Department of Cytology and Gynaecological Pathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India