Immune Homeostasis, 1st ed. 2024 Methods and Protocols Methods in Molecular Biology Series, Vol. 2782

This volume discusses different aspects of immune homeostasis regulation and its dysregulation during various diseases, including infections, cancers, neurodegenerative diseases, autoinflammation, autoimmune diseases, and immunodeficiency, along with immunological, genetic, cell biology, and molecular biology techniques designed to understand immune homeostasis at different stages. The chapters in this book cover topics such as T cell subsets; monocyte-myofibroblast immunomodulation in a 3D co-culture system; human peripheral blood mononuclear cells in patients with neurodegenerative diseases; and CRISPR-Cas9 induced gene-editing in primary human monocytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Immune Homeostasis: Methods and Protocols is a valuable resource for both novice and experts researchers interested in learning more about this important and developing field.  

Preface…

Table of Contents…

Contributing Authors…

1. Immune Homeostasis: A Novel Example of Teamwork

Vijay Kumar and John H. Stewart IV

2. Immune Homeostasis Maintenance through Advanced Immune Therapeutics to Target Atherosclerosis 

Shuo Geng, Yajun Wu, and Liwu Li

3. T Cell Subsets and Immune Homeostasis

Chuyu Wu, Mei-Ling Jiang, Tao Pang, and Cun-Jin Zhang

4. Post-Transcriptional Events Orchestrate Immune Homeostasis of CD8+T Cells

5. A Robust In Vitro Culture Model and Generation of Memory Monocytes

Jing Wang, Shuo Geng, Yajun Wu, and Liwu Li

6. A Robust In Vitro Co-Culture Model for Studying the Intercellular Communication of Neutrophils

RuiCi Lin, Yao Zhang, Shuo Geng, and Liwu Li

7. Studying Monocyte-Myofibroblast Immunomodulation in a 3D Co-Culture System

Avi Petroff

8. Endothelial Mitochondria-Associated Membranes (MAMs) Isolation by Percoll Step Gradients

Margaret Baldini, Cheng Zhang, and Jun Yu

9. Analysis of Human Peripheral Blood Mononuclear Cells in Patients with Neurodegenerative Diseases

Mary Dover, Melika Madani, Milan Fiala, and Ketema Paul

10. Modulation and Determination of the Status of Inflammasomes in Leishmania-Infected Macrophages 

Arunima Biswas, Anindita Bhattacharjee, and Pijush K. Das

Maria Elisabetta Federica Palamá, Maurizio Aiello, and Silvia Scaglione

12. Isolation and Single-Cell Transcriptomic Analysis of Murine Regulatory B Cells

Razan M. Alajoleen, Liwu Li, and Xin M. Luo

Zihao Li and Cun-Jin Zhang

14. Phenotypic and Functional Characterization of Memory CD4+ and CD8+ T Cells after Antigenic Stimulation 

Carolina do Prado Servian, Letícia Carrijo Masson, and Simon Gonçalves Fonseca

15. CRISPR-Cas9 Induced Gene-Editing in Primary Human Monocytes

Xiaofeng Liao and Liwu Li

16. Employing CRISPR-Cas9 to Enhance T Cell Effector Function

Julian J. Freen-van Heeren

17. Assessing Antigen-Specific T Cell Responses through IFN- Enzyme-Linked Immune Absorbent Spot (ELISpot) 

Julian J. Freen-van Heeren, Virginia Palomares Cabeza, David Cobeta Lopez, Domenique Kivits, Irma Rensink, Annelies W. Turksma, and Anja ten Brinke

Subject Index List…