Preface…
Table of Contents…
Contributing Authors…
1. Challenges in Developing Protein Secretion Assays at a Single-Cell Level
Yoshitaka Shirasaki and Osamu Ohara
2. To Count of Not to Count: ELISPOT Evaluation Revisited
Sylvia Janetzki
3. Essential Controls for ELISpot Assay
Jodi Hagen and Alex Kalyuzhny
4. Automatic Search of Spots and Color Classification in ELISPOT Assay
Sergey S. Zadorozhny and Nikolai N. Martynov
5. Four Color ImmunoSpot ® Assays for Identification of Effector T Cell Lineages
Jodi Hanson, Diana R. Roen, and Paul V. Lehmann
6. Detection of Cross-Reactive B Cells Using the FluoroSpot Assay
Peter Jahnmatz and Niklas Ahlborg
7. Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations
Diana R. Roen, Jodi Hanson, and Paul V. Lehmann
8. Detecting All Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assays
Richard Caspell and Paul V. Lehmann
9. Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot® Software Based on Center of Mass Distance Algorithm
Alexey Y. Karulin, Zoltán Megyesi, Richard Caspell, Jodi Hanson, and Paul V. Lehmann
10. Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X™ Suite
Zoltán Megyesi, Paul V. Lehmann, and Alexey Y. Karulin
11. B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells
Haw Hwai, Yi-Ying Chen, and Siang-Jong Tzeng
12. Identification of Novel Mycobacterial Targets for Murine CD4+T Cells by IFN ELISPOT
Alison J. Johnson, Steven C. Kennedy, Tony W. Ng, and Steven A. Porcelli
13. ELISPOT-Based “Multi-Color FluoroSpot” to Study Type-Specific and Cross-Reactive Responses in Memory B Cells after Dengue and Zika Virus Infections
Paulina Andrade, Josefina Coloma, and Eva Harris
14. Cultured ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses
Chandima Jeewandara, Graham S. Ogg, and Gathsaurie Neelika Malavige
15. Ex Vivo ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses
Gathsaurie Neelika Malavige
16. Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue Infection
Achala Indika Kamalasasa and Gathsaurie Neelika Malavige
17. Dendritic Cell-Based ELISpot Assay for Assessing T Cell-IFN- γ Responses in Human Peripheral Blood Mononuclear Cells to Dengue Envelope Proteins
Peifang Sun and Monika Simmons
18. Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccines
Bikash Sahay, Alek M. Aranyos, Andrew McAvoy, and Janet K. Yamamoto
19. Detection and Quantification of Influenza A/H1N1 Virus-Specific Memory B Cells in Human PBMCs using ELISpot Assay
Iana H. Haralambieva, Inna G. Ovsyannikova, Richard B. Kennedy, and Gregory A. Poland
