Food Allergens, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1592

This volume provides a collection of methodologies for basic research, clinical diagnosis, and treatment pertaining to food allergens, including food allergen production, purification, characterization, detection, quantification, and bioinformatics approaches to modern food allergen studies. The chapters in the book are divided into 4 parts: Part I discusses food allergen purification and production, and explores methods of producing recombinant food allergens in bacterial and yeast expression systems; Part II looks at allergen discovery, detection, and quantification covering 3 types of methods?DNA-, protein-, and cell-based methods; Part III focuses on allergenic epitope mapping; and Part IV talks about future developments concentrated around new concepts of allergenicity as an  outcome of protein and food matrix interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Food Allergens: Methods and Protocols is a valuable resource for immunologists, biochemists, molecular biologists, and medical doctors and students working in the food allergy field. This book is also useful for people in the food industry, legislators, food standard agencies, allergologists, pediatricians, and clinicians in the allergic diseases and immunology fields.

Preface…
Table of Contents…
Contributing Authors…

1: Overview of the Commonly used Methods for Food Allergens
Jing Lin and Marcos J.C. Alcocer

Part I Food Allergen Purification and Production

2: Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques
Barbara Cases, Carlos Pastor-Vargas, and Marina Perez-Gordo

3: Recombinant Allergen Production in E. Coli
Changqi Liu, LeAnna N. Willison, and Shridhar K. Sathe

4: Recombinant Allergens Production in Yeast
Maria Neophytou and Marcos J.C. Alcocer

Part II Food Allergen Discovery, Detection, and Quantification

5: 2D-Electrophoresis and Immunoblotting in Food Allergy
Galina Grishina, Luda Bardina, and Alexander Grishin

6: Two Dimensional Electrophoresis and Identification by Mass Spectrometry (MS)
Fernando de la Cyesta, Gloria Alvarez-Llamas, and Maria G. Barderas

7: Enzyme-Linked Immunosorbent Assay (ELISA)
George N. Konstantinou

8: Detection of Food Allergens by Taqman Real-Time PCR Methodology
Aina García, Raquel Madrid, Teresa García, Rosario Martín, and Isabel González

9: Detection of Food Allergens by Phage Displayed Produced Antibodies
Raquel Madrid, Silvia de la Cruz, Aina García, Rosario Martín, Isabel González, and Teresa García

10: Protein Microarray-Based IgE-Immunoassay for Allergy Diagnosis
Nuzul N. Jambari, XiaoWei Wang, and Marcos J.C. Alcocer

11: Basophil Degranulation Assay
Madhan Masilamani, Mohanapriya Kamalakannan, and Hugh A. Sampson

12: Use of Humanized RS-ATL8 Reporter System for Detection of Allergen-Specific IgE Sensitization in Human Food Allergy
Eman Ali Ali, Ryosuke Nakamura, and Franco H. Falcone

Part III Allergenic Epitope Mapping

13: Assessment of IgE Reactivity of β-Casein by Western Blotting after Digestion with Simulated Gastric Fluid
Sara Benedé, Rosina López-Fandiño, and Elena Molina

14: IgE Epitope Mapping using Peptide Microarray Immunoassay
Jing Lin and Hugh A. Sampson

15: T Cell Proliferation Assay: Determination of Immunodominant T Cell Epitopes of Food Allergens
Madhan Masilamani, Mariona Pascal, and Hugh A. Sampson

16: Tetramer Guided Epitope Mapping: A Rapid Approach to Identify HLA-Restricted T-Cell Epitopes from Composite Allergens
Luis Diego Archila and William W. Kwok

17: T-Cell Epitope Prediction
George N. Konstantinou

18: An Overview of Bioinformatics Tools and Resources in Allergy
Zhiyan Fu and Jing Lin

Part IV Methods Currently being Developed and Future Development

19: The Use of a Semi-Automated System to Measure Mouse Natural Killer T (NKT) Cell Activation by Lipid Loaded Dendritic Cells
Ashfaq Ghumra and Marcos J.C. Alcocer

20: Recent Advances in the Detection of Allergens in Foods
Silvia de la Crus, Inés López-Calleja, Rosario Martín, Isabel González, Marcos J.C. Alcocer, and Teresa García 

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras