Experimental and Translational Methods to Screen Drugs Effective Against Seizures and Epilepsy, 1st ed. 2021 Neuromethods Series, Vol. 167

This volume discusses the latest experimental and clinical approaches to study seizures and epilepsy. Chapters in this book cover detailed protocols for ex vivo and in vitro experimental models and widely-used in vivo (acute and chronic seizure) models in epilepsy research. Chapters also cover techniques to screen the effective leads for the treatment of refractory seizures of pharmacoresistant epilepsy and invertebrate, non-mammalian models. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. 

Cutting-edge and practical, Experimental and Translational Methods to Screen Drugs Effective Against Seizures and Epilepsy is a valuable resource for researchers, in both industry and academia, interested in learning about this important and developing field.

Series Preface…
Foreword…
Preface…
Table of Contents…
Contributing Authors…

Part I Models for Seizures and Epilepsy: An Overview

1. Models of Seizures and Epilepsy: Important Tools in the Discovery and Evaluation of Novel Epilepsy Therapies 
Wolfgang Löscher

Part II Ex Vivo and In Vitro Models

2. Protocol for Rodent Organotypic Hippocampal Slice Culture Model for Ex Vivo Monitoring of Epileptogenesis
Shabnam Ghiasvand, Jing Liu, Md. Joynal Abedin, and Yevgeny Berdichevsky

3. Methods for the Screening of New Chemical Entities for Deciphering Neuroinflammatory and Associated Pathways in Seizures: An In Vitro Perspective
Preeti Vyas, Rajkumar Tulsawani, and Divya Vohora

4.  Cellular Electrophysiological Methods to Decipher the Altered Synaptic Transmission Associated with Drug Resistant Epilepsy
Soumil Dey, Aparna Banerjee Dixit, Manjari Tripathi, P. Sarat Chandra, and Jyotirmoy Banerjee

Part III In Vivo Rodent Models 

5. Acute Seizure Tests Used in Epilepsy Research: Step-by-Step Protocol of the Maximal Electroshock Seizure (MES) Test, The Maximal Electroshock Seizure Threshold (MEST) Test, and the Pentylenetetrazole (PTZ)-Induced Seizure Test in Rodents
Katarzyna Socała and Piotr Wlaź

6. Procedures for Electrical and Chemical Kindling Models in Rats and Mice
Heidrun Potschka

7. Methods for the Induction of status epilepticus and Temporal Lobe Epilepsy in Rodents: The Kainic Acid and the Pilocarpine Model
Shreshta Jain, Nikita Nirwan, Fahim H. Pottoo, Nidhi Bharal Agarwal, and Divya Vohora 

8. Seizure Monitoring in Rodents
Edward H. Bertram 

Part IV Models for Refractory Seizures 

9. Protocol for 6Hz Corneal Stimulation in Rodents for Refractory Seizures
Razia Khanam and Divya Vohora

10. A Method to Induce Lamotrigine-Resistant Corneal Kindled Mice
Melissa Barker-Haliski

11. Protocol for Drug Screening with Quantitative Video-Electroencephalography in a Translational Model of Refractory Neonatal Seizures
Brennan J. Sullivan and Shilpa D. Kadam

Part V Invertebrate/Non-Mammalian Models 

12. Methods to Investigate Seizures and Associated Cognitive Decline using Zebrafish Model
Brandon Choo Kar Meng and Mohd. Farooq Shaikh

13. C. elegans as a Potential Model for Acute Seizure-Like Activity
Alistair Jones, Anthony G. Marson, Vincent T. Cunliffe, Graeme J. Sills, and Alan Morgan

Subject Index List…

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts