Circulating Tumor Cells, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1634
This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Circula
Dedication…
Acknowledgement…
Foreword…
Preface…
Table of Contents…
Contributing Authors…
1. Microfluidic Capture and Multiplex Immunofluorescence of Circulating Tumor Cells to Identify Cancer Origin
Chian-Hui Lai and Ying-Chih Chang
2. Microfluidic Separation of Circulating Tumor Cells Based on Size and Deformability
Emily S. Park, Simon P. Duffy, and Hongshen Ma
3. A Novel Microfluidic Device for Isolation of Circulating Tumor Cells from Pancreatic Cancer Blood Samples
Jose I. Varillas, Kangfu Chen, Jinling Zhang, Thomas J. George, Jr., and Z. Hugh Fan
4. Microfluidic-Based Enrichment and Retrieval of Circul
ating Tumor Cells for RT-PCR AnalysisPriya Gogoi, Saedeh Sepehri, Will Chow, Kalyan Handique, and Yixin Wang
5. Micro-Scale Laminar Vortices for High-Purity Extraction and Release of Circulating Tumor Cells
Soojung Claire Hur, James Che, and Dino Di Carlo
6. Separable Bilayer Microfiltration Device for Viable Label-free Enrichment of Circulating Tumor Cells
Sijie Hao, Merisa Nisic, Hongzhang He, Yu-Chong Tai, and Si-Yang Zheng
Siddarth Rawal, Zheng Ao, Ram H. Datar, and Ashutosh Agarwal
8. Fourier Ptychographic Microscopy for Rapid, High Resolution Imaging of Circul
ating Tumor Cells Enriched by MicrofiltrationAnthony Williams, Jaebum Chung, Changhuei Yang, and Richard J. Cote
9. Automated Microfluidic Filtration and Immunocytochemistry Detection System for Capture and Enumeration of Circulating Tumor Cells and Other Rare Cell Populations in Blood
Michael Pugia, Mark Jesus M. Magbanua, and John W. Park
10. Filter-Adapted Fluorescent In Situ Hybridization (FA-FISH) for Filtration-Enriched Circulating Tumor Cells
Marianne Oulhen, Emma Pailler, Vincent Faugeroux, and Françoise Farace
Nisha Kanwar and Susan J. Done
12. Capture and Genetic Analysis of Circulating Tumor
Cells Using a Magnetic Separation Device (Magnetic Sifter)Chin Chun Ooi, Seung-min Park, Dawson J. Wong, Sanjiv S. Gambhir, and Shan X. Wang
13. RareCyte® CTC Analysis Step 1: AccuCyte® Sample Preparation for the Comprehensive Recovery of Nucleated Cells from Whole Blood
Arturo B. Ramirez, Lance U’Ren, Danny Campton, David Stewart, Joshua J. Nordberg, Jackie L. Stilwell, and Eric P. Kaldjian
14. RareCyte ® CTC Analysis Step 2: Detection of Circulating Tumor Cells by CyteFinder® Automated Scanning and Semi-Automated Image Analysis
Jeffrey L. Werbin, Joshua J. Nordberg, Jay Tzucker, Paulina Varshavskaya, Jackie L. Stilwell, and Eric P. Kaldjian
15. RareCyte ® CTC Analysis Step 3: Using the CytePicker® Module for Individual Cell Retrieval and Subsequent Whole
Genome Amplification of Circulating Tumor Cells for Genomic AnalysisJackie L. Stilwell, Paulina Varshavskaya, Jeffrey L. Werbin, Joshua J. Nordberg, Arturo B. Ramirez, Steve Quarre, Jay Tzucker, Jennifer Chow, Brennan Enright, and Eric P. Kaldjian
16. Enumeration, Dielectrophoretic Capture, and Molecular Analysis of Circulating Tumor Cells
Stephanie S. Yee and Erica L. Carpenter
17.
18. Flow Cytometric Detection of Circulating Tumor Cells Using a Candidate Stem Cell Marker, p75 Neurotrophin
Receptor (p75NTR)< 19. Multispectral Imaging Analysis of Circulating Tumor Cells in Negatively-Enriched Peripheral Blood Samples
Brandon Miller, Maryam Lustberg, Thomas A. Summers, and Jeffrey J Chalmers
Zheng Ao and Xiaohe Liu
21. A Non-Invasive and Real-Time Method for Circulating Tumor Cell Detection: In Vivo Flow Cytometry
Xunbin Wei, Jian Zhou, Xi Zhu, Xinrong Yang, Ping Yang, and Qiyan Wang
22. EpCAM-Independent Enrichment and Detection of Viable CTCs Using the EPISPOT As
sayAlexandra Soler, Laure Cayrefourcq, Martine Mazel, and Catherine Alix-Panabières
23. Utilizing Matrigel Transwell Invasion Assay to Detect and Enumerate Circulating Tumor Cells
Xingtong Liu and Xiangwei Wu
24. Circulating Tumor Cells: Markers and Methodologies for Enrichment and Detection
M.J. Alvarez Cubero, J.A. Lorente, I. Robles-Fernandez, A. Rodriguez-Martinez, J.L. Puche, and M.J. Serrano
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 08-2018
Ouvrage de 306 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 168,79 €
Ajouter au panierDate de parution : 08-2017
Ouvrage de 306 p.
17.8x25.4 cm