Cancer Epigenetics for Precision Medicine , 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1856

This volume discusses certain epigenetic changes recognized in early carcinogenic lesions and different tumors, as well as factors that alter the epigenome and epigenetic profile such as diet, alcohol, immunity, circadian rhythm, and more. The chapters in this book further delve into this field and cover topics such as epigenome-based precision medicine in lung cancer; interplay between genetic and epigenetic changes in breast cancer subtypes; genetic regulation of PDCD1 gene in cancer immunology; and pyrosequencing methylation analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Cancer Epigenetics for Precision Medicine: Methods and Protocols is a valuable resource to help researchers and scientists identify these specific biomarkers and work towards the prevention, diagnosis, and prognosis of different cancers in the future.

Preface…

Table of Contents…

Contributing Authors…

Part I Early Epigenetic Changes and New Epigenetic Targets in Different Types of Cancers

1. Early Epigenetic Markers for Precision Medicine

2. Interplay between Genetic and Epigenetic Changes in Breast Cancer Subtypes

Ramona G. Dumitrescu

3. Role of Microbiome in Carcinogenesis Process and Epigenetic Regulation of Colorectal Cancer

Lulu Farhana, Hirendra N. Banerjee, Mukesh Verma, and Adhip PN Majumdar

4. Epigenome-Based Precision Medicine in Lung Cancer

Dong Hao Jin and Duk-Hwan Kim

5. Epigenetics in Hematological Malignancies 

N. Cruz-Rodriguez, AL Combita, and J. Zabaleta

6. MicroRNAs Role in Prostate Cancer

Ovidiu Balacescu, Ramona G. Dumitrescu, and Catalin Marian

Part II Contributing Environmental Factors to Epigenetic Changes Leading to Cancer Development

7. The Effects of Dietary Nutrients on Epigenetic Changes in Cancer

Nicoleta Andreescu, Maria Puiu, and Mihai Niculescu

8. Diet, Microbiome, and Epigenetics in the Era of Precision Medicine

Gabriela Riscuta, Linda Duffy, Dan Xi, Dudith Pierre-Victor, Pamela Starke-Reed, and Jag Khalsa

9. Alcohol-Induced Epigenetic Changes in Cancer

Ramona G. Dumitrescu

10. Epigenetic Basis of Circadian Rhythm Disruption in Cancer

Edyta Reszka and Shanbeh Zienolddiny

11. Epigenetic Changes of the Immune System with Role in Tumor Development

Irina Daniela Florea and Christina Karaoulani

12. DNA Methylation as a Biomarker of Aging in Epidemiologic Studies

Unhee Lim and Min-Ae Song

13. Challenges and Opportunities in Social Epigenomics and Cancer

Krishna Banaudha, Vineet Kumar, and Mukesh Verma 

Part III Methods Used in Cancer Epigenomics 

14. Epigenetic and Genetic Regulation of PDCD1 Gene in Cancer Immunology

Alok Mishra and Mukesh Verma 

15. Methylation and Micro RNA Profiling to Understand Racial Disparities of Prostate Cancer

Hirendra Nath Banerjee, William Kahan, Vineet Kumar, and Mukesh Verma

16. Analysis of DNA Hypermethylation in Pancreatic Cancer using Methylation Specific PCR and Bisulfite Sequencing

Bin Liu and Christian Pilarsky

17. Pyrosequencing Methylation Analysis

Matthew Poulin, Jeffrey Y. Zhou, Liying Yan, and Toshi Shioda

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts