Biomolecular EPR Spectroscopy

Comprehensive, Up-to-Date Coverage of Spectroscopy Theory and its Applications to Biological Systems

Although a multitude of books have been published about spectroscopy, most of them only occasionally refer to biological systems and the specific problems of biomolecular EPR (bioEPR). Biomolecular EPR Spectroscopy provides a practical introduction to bioEPR and demonstrates how this remarkable tool allows researchers to delve into the structural, functional, and analytical analysis of paramagnetic molecules found in the biochemistry of all species on the planet.

A Must-Have Reference in an Intrinsically Multidisciplinary Field

This authoritative reference seamlessly covers all important bioEPR applications, including low-spin and high-spin metalloproteins, spin traps and spin lables, interaction between active sites, and redox systems. It is loaded with practical tricks as well as do?s and don?ts that are based on the author?s 30 years of experience in the field. The book also comes with an unprecedented set of supporting software designed with simple graphical user interfaces that allow readers to tackle problems they will likely encounter when engaged in spectral analysis.

Breaking with convention, the book broaches quantum mechanics from the perspective of biological relevance, emphasizing low-symmetry systems. This is a necessary approach since paramagnets in biomolecules typically have no symmetry. Where key topics related to quantum mechanics are addressed, the book offers a rigorous treatment in a style that is quick-to-grasp for the non expert. Biomolecular EPR Spectroscopy is a practical, all-inclusive reference sure to become the industry standard.

BASICS. Introduction. The Spectrometer. The Sample. Experimental Key Parameters. Resonance Condition. Analysis. THEORY. Energy Matrices. Biological Spin Hamiltonians. Conformational Distributions. SPECIFIC EXPERIMENTS. Aqueous Solutions. Interactions. High Spins Revisited. Black Box Experiments. Strategic Considerations. References. Index.

Wilfred Raymond Hagen