Basic Techniques in Biochemistry, Microbiology and Molecular Biology, 1st ed. 2020 Principles and Techniques Springer Protocols Handbooks Series

Section 1:

(A) Equipments

i. BOD incubators

ii. Laminar flow

iii. Biosafety Cabinets

iv. Aseptic hood

v. Autoclave

vi. Hot air sterilizer

vii. Deep freezer

viii. Refrigerator

ix. Microscopy

x. Colony counter

xii. Turbidometer

xiii. Colorimeter

xiv. Spectrophotometer

xv. PCR

xvi. ELISA reader

(B) Practical Molecular Biology 

1. Enzyme assay: qualitative and quantitative (protease, amylase, penicillinase, pectinolytic enzyme, chitinase, keratinase)

2. Antimicrobial sensitivity assay 

3. Extraction of DNA and RNA: Plant cells, Animal cells and Microorganism

4. Estimation of DNA by diphenylamine reaction

5. Estimation of RNA using orcinol method

6. Determination of melting temperature of DNA

7. Quantitative estimation of DNA and RNA

8. Purification and bioassay of interleukin 1 and 2 (IL 1 and IL 2)

9. Quantitative determination of TNF alpha and Interleukin 6 in the given sample

10. Quantitative determination of amount of mono and oligo nucleosomes in the cytosol of cell extract sample after an induced cell death.

(C) Practical Microbiology

1. Sterilization of glassware, preparation of media and its sterilization

2. Sub culturing of bacteria, fungi and actinomycetes.

3. Preservation of microorganisms: stabs, slants, lyophilization, cryopreservation 

4. Staining methods: Simple, negative, Gram staining, Acid-fast staining, cell wall, capsule, flagella, nuclear material, cytoplasm, filamentous fungi, mycoplasma

5. Isolation and purification of microorganisms: streaking, pour plate, spread plate, serial dilution, Warcup method, baiting technique, enrichment culture

6. Isolation of coprophilous fungi 

7. Isolation of microorganisms from air, water 

8. Isolation of anaerobic microorganisms 

9. Isolation of extremophiles: thermophiles and psychrophiles

10. Microbiological assay of antibiotics by cup plate method

11. Motility testing: hanging drop method and stab

12. Sterility testing of pharmaceuticals

13. Physical, Chemical and Bacteriological analysis of water

14. Biochemical identification of microorgamism: IMViC, oxidative fermentation, carbohydrate and nitrogen source                      utilization

15. Immobilization of microorganisms and enzymes

Section 2:

1. Qualitative systematic analysis of carbohydrates (Glucose, Fructose, Lactose, Maltose, Sucrose and starch).

2. Protein analysis in food.

3. Identification tests for Proteins (Albumin and Casein).

4. Quantitative analysis of reducing sugars by 3, 5-Dinitrosalicylic acid (DNSA method). 

5. Quantitative analysis of Proteins by various methods including Biuret.

6. Qualitative analysis of urine for abnormal constituents.

7. Determination of blood creatinine.

8. Determination of blood sugar.

9. To perform oral glucose tolerance test.

10. Determination of serum total cholesterol.

12. Study of enzymatic hydrolysis of starch.

13. Determination of Salivary amylase activity.

14. Study the effect of Temperature on Salivary amylase activity.

15. Study the effect of substrate concentration on salivary amylase activity.

16. Analysis of Butter.

17. Analysis of Milk.

18. TLC and paper chromatography of amino acid and carbohydrates.

19. Fat characterization

20. Analysis of bread.

AAKANCHHA JAIN is an Associate Professor at Bhagyoday Tirth Pharmacy College, Sagar, India. She possesses a throughout first class career. She has to her credit 11 national awards including Nagarjuna Award 2010, Gour Samman 2010, Ranbaxy Science Scholar 2012, and MP Young Scientist Award 2018. She is a Zealous Teacher and Researcher. She has published 23 review and research articles in peer-reviewed high-indexed journals. She was formerly INSPIRE DST Research Fellow at the Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar, India, from where she has completed her PhD, MPharm, and BPharm. She has also published two invited book chapters (Wiley, Pan Stanford) covering wide aspects of biotechnology demonstrating her innovation and creativity in delivering lasting results in the field. She has examined many aspects of drug delivery through nanoparticles in her MPharm and PhD. Her main contributions have been in the field of formulation and characterization of novel drug delivery systems, transdermal drug delivery, and anticancer drug delivery systems.

RICHA JAIN is a Senior Scientist, working in the Centre for Scientific Research and Development, People’s University, Bhopal, India, possesses more than 14 years of research experience in the area of molecular biology, animal cell culture, microbiology, bioremediation, and industrial and therapeutic enzymes. Presently, she is working on two projects: (a) therapeutic enzymes arginases (anticancer) and (b) bioremediation of plastic waste. She has published 21 research papers with 81 citations in research journals of high repute. Among several feathers on her cap includes Best Paper Award for the presentation of her paper on “Bioinformatics: Importance of Proteomics and Genomics” during the National Conference on “New Frontiers of Sciences” National Science Day Celebrations 2003, Dr. Hari Singh Gour University, Sagar, 28 February 2003; All India Young Scientist Award for