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Allergy (2nd Ed., 2nd ed. 2019) Methods and Protocols Methods in Molecular Biology Series, Vol. 2020

Langue : Anglais

Coordonnateurs : Lympany Penny, Jones Meinir G.

Couverture de l’ouvrage Allergy
This second edition volume expands on the previous edition with new insights into the molecular mechanisms involved in allergy through the use of various protocols that cover disciplines like allergy, immunology, cell biology, and histology. The chapters in this book cover topics such as characterization of allergens using western blotting and mass spectrometry, collecting and sampling airborne allergens, IgG antibodies, identification and characterization of allergen specific T cells, and the role of antigen presenting cells in T cell proliferation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Allergy: Methods and Protocols, Second Edition is a valuable resource for researchers interesting in beginning or continuing their research the study of allergies. 

Table of Contents…
Contributing Authors…

1.  Understanding of the Molecular Mechanisms of Allergy
Meinir Jones

2. T-Cell: Primary Culture from Peripheral Blood
Monika Raulf

3. Measurement of Allergen-Specific Inhibitory Antibody Activity
Mohamed H. Shamji and James N. Francis

4. Immunoelectrophoresis for the Characterization of Allergen Extracts
Gitte Nordskov Hansen and Jørgen Nedergaard Larsen

5. Standardization of Allergen Extracts
Jørgen Nedergaard Larsen and Sten Dreborg

6. Quantifying Human Innate Cytokine and Chemokine Responses Ex Vivo via Pattern Recognition Receptor Stimulation
Natascha Fitch, Stephanie J. Marshall, William P. Stefura, Rishma Chooniedass, Allan B. Becker, and Kent T. HayGlass

7. Improved Methods for Quantifying Human Chemokine and Cytokine Biomarker Responses: Ultrasensitive ELISA and Meso Scale Electrochemiluminescence Assays
William P. Stefura, Caroline Graham, Larisa Lotoski, and Kent T. HayGlass

8. Development of Mould Antigen-Specific Enzyme-Linked Immunosorbent Assays (ELISA) to Quantify Airborne Antigen Exposure
Eva Zahradnik and Monika Raulf

9. Monocyte-Derived Dendritic Cells as Antigen Presenting Cells in T-Cell Proliferation and Cytokine Production
Sun-sang J. Sung

10. The Use of Dual Cell Tracker Dye Staining for the Identification and Characterization of Peanut Specific T-Cell Subsets
Cecilia Dunsterville, Alick C. Stephens, Gideon Lack, and Victor Turcanu

11. The Use of pMHCII Tetramer Technology for the Identification and Characterization of CD4+ T Cells
Erik Wambre

12. Isolation and Characterization of Lymphocytes from Human Mucosal Biopsies
Nadine E.G. Upton, Thomas S. Hayday, and Louisa K. James

13. Development of a Dipstick Assay for Soy Allergens: Inexpensive Detection to Control Allergen Exposure
Daniel Álvarez-Simón and Susana Gómez-Ollés

14. Novel Method for the Purification of House Dust Mite Allergen Der p 1 and Its Use in Structure-Based Chemical Design of Novel Inhibitors
Jihui Zhang and Clive Robinson

15. Measurement of Specific IgG Anti-Fel d 1 Antibodies
Meinir Jones

16. Measurement of Specific IgG4 Anti-Mouse Urine Antibodies
Jennifer Canizales

17. Preparation of Samples for a Mass Spectrometry Based Method to Identify Allergenic Proteins
Mary Brian
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts

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