Targeted Drug Delivery, 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1831

This volume looks at the latest techniques used to enable therapeutic molecules to be targeted for site-specific delivery. The chapters in this book discuss topics such as approaches to express biologically derived and synthetic nanocarriers; strategies to assist in nanocarrier targeting to specific cells and tissues; and methods for evaluating delivery and efficiency for new classes of agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Targeted Drug Delivery: Methods and Protocols is a valuable resource for anyone interested in understanding the diversity of methods used in this evolving field.

Table of Contents…

Contributing Authors…

Part I Drug Carrier Design

1. Synthesis of Cationic Polymer Libraries for Gene Delivery using Diglycidyl Ethers

Jacob Elmer, Thrimoorthy Potta, and Kaushal Rege

2. Generation of Ultra-Small PLGA Nanoparticles by Sequential Centrifugation

Xingwang Wu, Jiangbing Zhou, and Toral R. Patel

3. Construction of Bacteria-Based Cargo Carriers for Targeted Cancer Therapy

Mahama A. Traore, Ali Sahari, and Bahareh Behkam

4. Production of Extracellular Vesicles Loaded with Therapeutic Cargo

Tek N. Lamichhane and Steven M. Jay

5. Delivery of Cytotoxic Mesenchymal Stem Cells with Biodegradable Scaffolds for Treatment of Postoperative Brain Cancer

Kevin T. Sheets, Juli R. Bagó, and Shawn D. Hingtgen

6. Nanoparticles for Targeted Drug Delivery to Cancer Stem-Like Cells and Tumor

Hai Wang and Xiaoming He

Part II Passive and Active Targeting Methods

7. Exploiting Phage Display for Development of Novel Cellular Targeting Strategies

8. CD44 Targeted Lipid Nanoparticles for MicroRNA Therapy

Stephen L. Hayward and Srivatsan Kidambi

9. Ultrasound and Microbubble-Mediated Blood-Brain Barrier Disruption for Targeted Delivery of Therapeutics to the Brain

Meaghan A. O'Reilly and Kullervo Hynynen

10. In Vitro Validation of Targeting and Comparison to Mathematical Modeling

Jill M. Steinbach-Rankins and Michael R. Caplan

Part III Measuring Delivery and Efficacy

11. Enzyme-Linked Immunosorbent Assay to Quantify Targeting Molecules on Nanoparticles

Rachel S. Edelstein, Jilian R. Melamed, and Emily S. Day

12. Tunable Collagen Microfluidic Platform to Study Nanoparticle Transport in the Tumor Microenvironment

Matthew R. DeWitt and M. Nichole Rylander

13. Utilizing the Lung as a Model to Study Nanoparticle Based Drug Delivery Systems

Dylan K. McDaniel, Veronica M. Ringel-Scaia, Sheryl L. Coutermarsh-Ott, and Irving C. Allen

14. Non-Enzymatic Tissue Homogenization for Biodistribution Analysis

Danielle M. DiPerna, Alesia V. Prakapenka, Eugene P. Chung, and Rachael W. Sirianni

15. Methods and Study Designs for Characterizing the Pharmacokinetics and Pharmacodynamics of Carrier-Mediated Agents

Allison N. Schorzman, Andrew T. Lucas, John R. Kagel, and Willliam C. Zamboni