Pre-Clinical Models, 1st ed. 2019 Techniques and Protocols Methods in Molecular Biology Series, Vol. 1916

This volume details reviews and protocols on the development and analysis of both cellular and animal-based pre-clinical models in a number of medical areas, including metabolic disorders, longevity, cancer, heart disease and psychiatric disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Pre-Clinical Models: Techniques and Protocols aims to provide methods that describe the context of specific disease or therapeutic areas.

Part I: Reviews

1.                  A Guide to Mass Spectrometry-based Quantitative Proteomics                          

Bradley J. Smith, Daniel Martins-de-Souza, and Mariana Fioramonte

Keiko Iwata

3.                  Monosodium Glutamate (MSG)-induced Animal Model of Type 2 Diabetes

Part II: Protocols

4.                  The Elevated Plus Maze Test for Measuring Anxiety-like Behaviour in Rodents

Ann-Katrin Kraeuter, Paul C. Guest, and Zoltán Sarnyai

5.                  The Forced Swim Test for Depression-like Behaviour in Rodents

Ann-Katrin Kraeuter, Paul C. Guest, and Zoltán Sarnyai

6.                  Object Burying Test for Assessment of Obsessive Compulsive Behaviours in Mice

Ann-Katrin Kraeuter, Paul C. Guest, and Zoltán Sarnyai

7.                  The Nest Building Test in Mice for Assessment of General Well-being

Ann-Katrin Kraeuter, Paul C. Guest, and Zoltán Sarnyai

Ann-Katrin Kraeuter, Paul C. Guest, and Zoltán Sarnyai

9.                  The Open Field Test for Measuring Locomotor Activity and Anxiety-Like Behaviour

10.              The Y-maze for Assessment of Spatial Working and Reference Memory in Mice

Ann-Katrin Kraeuter, Paul C. Guest, and Zoltán Sarnyai

Gabriela Seabra, Valéria de Almeida, and Daniel Martins-de-Souza

12.              Monitoring Age-related Changes in the Lactate/pyruvate Ratio using a Colorimetric Assay in a C. elegans Model of Increased Lifespan

Sumino Yanase, Kayo Yasuda, and Naoki Ishii

Frans Alexander Jacobs, Mari van de Vyver, and William Frank Ferris

14.              Characterization of an Animal Model of Autism and Social Interaction

15.              Assaying Reproductive Capacity in Female Rodents

16.              Experimental Model of Hindlimb Suspension-Induced Skeletal Muscle Atrophy in Rodents

Gabriel Nasri Marzuca-Nassr, Kaio Fernando Vitzel, Gilson Masahiro Murata, José Luis Márquez, and Rui Curi

Yuji Takeda, Hironobu Asao, and Ichiro Wakabayashi

18.              Characterization of the db/db Mouse Model of Type 2 Diabetes

Paul C Guest and Hassan Rahmoune

19.              Characterization of the Goto-Kakizaki (GK) Rat Model of Type 2 Diabetes

Paul C. Guest

Paul C. Guest

21.              Preparation of Islets from Rat Pancreas and Assessment of Islet Function

22.              Cryopreservation of Red Blood Cells

Noha AS Alotaibi, Nigel KH Slater, and Hassan Rahmoune

23.              Molecular Characterization of Human Leukemia 60 (HL-60) Cells as a Model of Acute Myelogenous Leukemia Post Cryo-preservation

Noha AS Al-Otaibi, Juliana S Cassoli, Nigel KH Slater, and Hassan Rahmoune

24.              Reprogramming of Human Melanocytes and Melanoma cells with Yamanaka Factors

Hadiseh Taheri, Umut Cagin, and Açelya Yilmazer

25.              The Use of 3T3-L1 Murine Preadipocytes as a Model of Adipogenesis

Eleanor Cave and Nigel J Crowther

26.              Generation of Human Pyruvate Carboxylase Knockout Cell Lines Using Retrovirus Expressing Short Hairpin RNA and CRISPR-Cas9 as Models to Study its Metabolic Role in Cancer Research

Khanti Rattanapornsompong, Jarunya Ngamkham, Tanit Chavalit, and Sarawut Jitrapakdee

27.              The use of Primary Hepatocytes in Assessment of Drug Safety and Toxicity

Paul C. Guest

28.              OVA-induced Allergic Airway Inflammation Mouse Model

Mateus Casaro, Vanessa R. Souza, Fernando A. Oliveira, and Caroline M. Ferreira

29.              Description of Ovariectomy Protocol in Mice

Vanessa R. Souza, Eduardo Mendes, Mateus Casaro, Ana Tada F.B. Antiorio, Fernando A. Oliveira, and Caroline M. Ferreira

30.              A Rat Eye Lens Model of Cataract Formation

Paul C. Guest

31.              A Complete Proteomic Workflow to Study Brain-Related Disorders via Postmortem Tissue

Guilherme Reis-de-Oliveira, Mariana Fioramonte, and Daniel Martins-de-Souza

32.              Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 β-Cell Line– A Pre-clinical Model for Type 2 Diabetes

Jordan Rowlands, Nikita Walz, Joanne E. Rowles, Kevin N. Keane, Rodrigo Carlessi, and Philip Newsholme

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts