High-Throughput Screening Assays in Toxicology, 1st ed. 2016 Methods in Molecular Biology Series, Vol. 1473

Part I: In Vitro Toxicological High-Thoughput Screening Methods

1. Monitoring Ligand-Activated Protein-Protein Interactions Using Bioluminescent Resonance Energy Transfer (BRET) Assay

            Carlos Coriano, Emily Powell, and Wei Xu

2. Mitochondrial Membrane Potential Assay

            Srilatha Sakamuru, Matias S. Attene-Ramos, and Menghang Xia

3. Using β-Lactamase and NanoLuc Luciferase Reporter Gene Assays to Identify Inhibitors of the HIF-1 Signaling Pathway

4. Quantitative High-Throughput Luciferase Screening in Identifying CAR Modulators

            Caitlin Lynch, Jinghua Zhao, Hongbing Wang, and Menghang Xia

5. Transactivation and Coactivator Recruitment Assays for Measuring Farnesoid X Receptor Activity

            Chia-Wen (Amy) Hsu, Jinghua Zhao, and Menghang Xia

6. Cell-Based Assay for Identifying the Modulators of Antioxidant Response Element Signaling Pathway

            Jinghua Zhao, Sunita J. Shukla, and Menghang Xia

7. Study Liver Cytochrome P450 3A4 Inhibition and Hepatotoxicity Using DMSO-Differentiated HuH-7 Cells

            Yitong Liu

8. Determination of Histone H2AX Phosphorylation in DT40 Cells

            Kana Nishihara, Sampada A. Shahane, and Menghang Xia

9. High-Throughput and High Content Micronucleus Assay in CHO-K1 Cells

Part II: In Vivo Toxicological High-Thoughput Screening Methods

10. Better, Faster, Cheaper: Getting the Most Out of High-Throughput Screening with Zebrafish

            Lisa Truong, Michael T. Simonich, and Robert L. Tanguay

11. Fast Functional Germline and Epigenetic Assays in the Nematode Caenorhabditis elegans

            Zachary Lundby, Jessica Camacho, and Patrick Allard

Part III: In Silico High-Thoughput Screening Toxcity Data Analysis

12. A Quantitative High-Throughput Screening Data Analysis Pipeline for Activity Profiling

            Ruili Huang

13. Correction of Microplate Data from High-Throughput Screening

            Yuhong Wang and Ruili Huang

14. CurveP Method for Rendering High-Throughput Screening Dose-Response Data into Digital Fingerprints

            Alexander Sedykh

15. Accounting Artifacts in High-Throughput Toxicity Assays

            Jui-Hua Hsieh

16. Accessing the High-Throughput Screening Data Landscape

            Daniel P. Russo and Hao Zhu

17. Curating and Preparing High Throughput Screening Data for Quantitative Structure Activity Relationship Modeling

            Marlene T. Kim, Wenyi Wang, Alexander Sedykh, and Hao Zhu

Includes cutting-edge methods and protocols for HTS assays to examine toxicity

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras