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Experimental Cholestasis Research, 1st ed. 2019 Methods in Molecular Biology Series, Vol. 1981

Langue : Anglais

Coordonnateur : Vinken Mathieu

Couverture de l’ouvrage Experimental Cholestasis Research
This detailed book provides a state-of-the-art compilation of protocols relevant for the experimental study of cholestasis, each written by internationally recognized research teams that have built up long-standing expertise regarding one or more aspects of experimental cholestasis research. The first half of the collection examines methods targeted towards studying established and new mechanisms of cholestasis, while the second half explores experimental models and tools to investigate mechanisms of cholestasis and/or to predict cholestatic properties of drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Experimental Cholestasis Research serves as an ideal guide for basic and applied researchers pursuing this vital area of research in pharmacology and toxicology.

1. Mechanisms of Drug-Induced Cholestasis

            Eva Gijbels and Mathieu Vinken


2. LC-MS/MS Analysis of Bile Acids in In Vitro Samples

            Debbie Dewaele, Pieter Annaert, and Eef Hoeben


3. Pipe-3D: A Pipeline Based on Immunofluorescence, 3D Confocal Imaging, Reconstructions, and Morphometry for Biliary Network Analysis in Cholestasis

            Amruta Damle-Vartak, Brigitte Begher-Tibbe, Georgia Gunther, Fabian Geisler, Nachiket Vartak, and Jan G. Hengstler


4. Vesicle-Based and Hepatocyte-Based Assays for Identification of Drug Candidates Inhibiting BSEP Function

            Pieter Van Brantegem, Neel Deferm, Bing Qi, Tom De Vocht, and Pieter Annaert


5. The C-DILI™ Assay:  An Integrated In Vitro Approach to Predict Cholestatic Hepatotoxicity

            Jonathan P. Jackson and Kenneth R. Brouwer


6. In VivoMeasurement of Hepatic Drug Transporter Inhibition with Radiolabeled Bile Acids

            Stef De Lombaerde, Sara Neyt, Christian Vanhove, and Filip De Vos


7. Measuring the Impact of Bile Acids on the Membrane Order of Primary Hepatocytes and Isolated Mitochondria by Fluorescence Imaging and Spectroscopy

            Tânia Sousa, Rui E. Castro, Ana Coutinho, Cecília M.P. Rodrigues, Manuel Prieto, and Fábio Fernandes


8. The Role and Study of Mitochondrial Impairment and Oxidative Stress in Cholestasis

            Reza Heidari and Hossein Niknahad


9. Measuring Apoptosis and Necrosis in Cholestatic Liver Injury

            Benjamin L. Woolbright and Hartmut Jaeschke


10. Necrosome Formation and Necroptosis in Experimental Cholestasis

            Marta Bento Afonso and Cecília Maria Pereira Rodrigues


11. Proteomics in Primary Biliary Cholangitis

            You Li, Ruqui Tang, Patrick S.C. Leung, Eric Gershwin, and Xiong Ma


12. Transcriptomics Analysis of Cholestatic Compounds In Vitro

            Céline Parmentier, Philippe Couttet, Marianne Uteng, Armin Wolf, and Lysiane Richert


13. Systems Microscopy of Stress Response Pathways in Cholestasis Research

            Johannes P. Schimming, Bas ter Braak, Marije Niemeijer, Steven Wink, and Bob van de Water


14. Mouse Models for Diseases in the Cholangiocyte Lineage

            Noémi Van Hul, Urban Lendahl, and Emma R. Andersson


15. Common Bile Duct Ligation as Model for Secondary Biliary Cirrhosis

            Sanne Van Campenhout, Hans Van Vlierberghe, and Lindsey Devisscher


16. 3,5-Diethoxycarbonyl-1,4-Dihydrocollidine Diet: A Rodent Model in Cholestasis Research

            Elisa Pose, Pau Sancho-Bru, and Mar Coll


17. A Rotavirus-Induced Mouse Model to Study Biliary Atresia and Neonatal Cholestasis

            Sujit K. Mohanty, Bryan Donnelly, Haley Temple, and Gregory M. Tiao


18. Zebrafish as a Model to Study Cholestatic Liver Diseases

            Duc-Hung Pham and Chunyue Yin


19. Set-Up and Use of HepaRG Cells in Cholestasis Research

            Christiane Guguen-Guillouzo and André Guillouzo


20. Organotypic 3D HepaRG Liver Model for Assessment of Drug-Induced Cholestasis

            Sreenivasa C. Ramaiahgari and Stephen S. Ferguson


21. Establishment of Sandwich Cultures of Primary Human Hepatocytes

            Eva Gijbels, Tamara Vanhaecke, and Mathieu Vinken


22. Detection of Drug-Induced Cholestasis Potential in Sandwich-Cultured Human Hepatocytes

            Neel Deferm, Lysiane Richert, Pieter Van Brantegem, Tom De Vocht, Bing Qi, Peter de Witte, Thomas Bouillon, and Pieter Annaert


23. Ex Vivo Model in Cholestasis Research

            Ruby E.H. Karsten, Dorenda Oosterhuis, Louise A. van Wijk, and Peter Olinga


24. Patient-Derived Organoids from Human Bile: An In Vitro Method to Study Cholangiopathies

            Carol J. Soroka, David N. Assis, and James L. Boyer


25. Use of Biliary Organoids in Cholestasis Research

            Fotios Sampaziotis, Olivia Tysoe, Teresa Brevini, and Ludovic Vallier


26. In Silico Approaches to Predict Drug-Transporter Interaction Profiles: Data Mining, Model Generation, and Link to Cholestasis

            Sankalp Jain and Gerhard F. Ecker

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

Date de parution :

Ouvrage de 438 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

179,34 €

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