Drug Target miRNA, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1517
This volume provides a concise and technical discussion of recently developed approaches to overcome challenges, such as pharmacodynamics and pharmacokinetics difficulties, in miRNA drug discovery. These strategies cover anti-sense agents targeting miRNA that are applied in advanced formulations or are chemically optimized to increase delivery; small molecule miRNA modulators to overcome anti-sense agents? limitations; general enhancers of miRNA maturation; and Argonaute 2 protein and its pharmacokinetic parameters. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Drug Target miRNA: Methods and Protocols is a valuable resource for anyone interested in the ever-evolving field of miRNA drug discovery.
PART I Reviews
1. MiRNA Targeting Drugs: The Next Blockbusters?
Marco F. Schmidt
PART II Protocols
2. Functional Analysis of miRNA using DIANA Tools Online Suite
Ionnis S. Vlachos and Artemis G. Hatzigeorgiou
3. Non-Nucleotide Modification of Anti-miRNA Oligonucleotides
Kim A. Lennox, Christopher A. Vakulskas, and Mark A. Behlke
4. Quantification of Oligonucleotide Association with miRNA-Argonaute Complexes In Vitro
Dimitrios G. Zisoulis
Dimitrios G. Zisoulis
John R. Androsavich
John R. Androsavich
Jane Zhao and Andreas G. Bader
Geneviève Pépin, Jonathan Ferrand, and Michael P. Gantier
Duc D. Vo and Maria Duca
Vinita Periwal and Vinod Scaria
Yangchao Chen
Christopher L. Haga, Sai Pradeep Velagapudi, Jessica L. Childs-Disney, Jacqueline Strivelli, Matthew D. Disney, and Donald G. Phinney
Johannes Danner, Balagopal Pai, Ludwig Wankerl, and Gunter Meister
Teresa Bellissimo, Silvia Masciarelli, Elena Poser, Ilaria Genovese, Alberto Del Rio, Gianni Colotti, and Francesco Fazi
Elena Poser, Ilaria Genovese, Silvia Masciarelli, Teresa Bellisimo, Francesco Fazi, and Gianni Colotti
Marco F. Schmidt, Oliver Korb, and Chris Abell
Sarah Willkomm and Tobias Restle
Sarah Willkomm, Adrian Zander, and Diana Grohmann
Myung Hyun Jo and Sungchul Hohng
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Ouvrage de 319 p.
17.8x25.4 cm
Date de parution : 07-2018
Ouvrage de 319 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 116,04 €
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