The Resolution Revolution: Recent Advances In cryoEM Methods in Enzymology Series
Auteur : CROWTHER R.
cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging.
1. Direct Electron Detectors - Methods in Enzymology (2016) Greg McMullan, Abdul R. Faruqi and Richard Henderson 2. Specimen Behavior in the Electron Beam Robert M. Glaeser 3. Specimen Preparation for High-Resolution Cryo-EM Christopher J. Russo and Lori A. Passmore 4. Strategies for Automated Cryoem Data Collection Using Direct Detectors Anchi Cheng, Yong Zi Tan, Venkat Dandey, Clinton S. Potter and Bridget Carragher 5. Processing of Cryo-EM Movie Data Zev A. Ripstein and John L. Rubinstein 6. Processing of Structurally Heterogeneous Cryo-EM Data in RELION Sjors H.W. Scheres 7. Single Particle Refinement and Variability Analysis in EMAN2.1 Steven J. Ludtke 8. Frealign: An Exploratory Tool for Single Particle Cryo-EM Nikolaus Grigorieff 9. Testing the Validity of Single Particle Maps at Low and High Resolution Peter B. Rosenthal 10. Tools for Model Building and Optimization into Near Atomic Resolution Electron Cryo-Microscopy Density Maps Frank DiMaio and Wah Chiu 11. Refinement of Atomic Structures Against CryoEM Maps Garib N. Murshudov 12. Cryo-EM Structure Determination Using Segmented Helical Image Reconstruction Simon A. Fromm and Carsten Sachse 13. Cryo-Electron Tomography and Subtomogram Averaging William Wan and John A.G. Briggs
- Continues the legacy of this premier serial with quality chapters authored by leaders in the field
- Covers research methods that determine the structures of biological molecules, a vital step for understanding their function
- Contains the technical developments underpinning the advances of cryoEM and captures the exciting insights that have resulted
Date de parution : 08-2016
Ouvrage de 486 p.
15x22.8 cm
Thèmes de The Resolution Revolution: Recent Advances In cryoEM :
Mots-clés :
Algorithm; Asymmetry; Automated data acquisition; Beam-induced movement; Camera; Classification; CMOS; Contrast transfer function; Cryo-electron microscopy; Cryo-electron tomography; cryoEM; CryoEM; Cryo-EM; CryoEM map-derived model; cryospi1-EMspi1; Crystal; Crystallography; Data collection strategies; Data processing; Databases; DDD; Diffraction; Direct detector device; Direct detectors; Direct Electron; Direct electron detector; Direct electron detectors; DQE; Electron; Electron microscopy; EMDataBank; EMDB; EMPIAR; Energy deposited; FEI; Gatan; Helical assemblies; Helical symmetry; High resolution; High-resolution; Image processing; In situ structural biology; Masking; MicroED; Model optimization; Model validation; Motion correction; Movie; MTF; Near-atomic resolution; Protein; Protein Data Bank; Radiation dose; Refinement; Segmented helical reconstruction; Sensor; Single particle methods; Single-particle helical reconstruction; Specimen charging; Specimen damage; Specimen motion; Subtomogram averaging; Throughput; Tobacco mosaic virus; Validation
