Fibrous Proteins, 1st ed. 2021 Design, Synthesis, and Assembly Methods in Molecular Biology Series, Vol. 2347

Part I: Introduction to Fibrous Proteins

1. The Structure of Animal Silks

            Wenwen Zhang and Yimin Fan

2. The Structure of Collagen

            Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, and Jing Ren

3. The Structure of Elastin

            Yuelong Xiao, Shengjie Ling, and Ying Pei

4. The Structure of Resilin

            Yuelong Xiao, Shengjie Ling, and Ying Pei

5. The Structure of Keratin

            Wenwen Zhang and Yimin Fan

Part II: Methods in Synthesis of Fibrous Proteins

6. General Methods to Produce and Assemble Recombinant Spider Silk Proteins

            Na Kong

7. Self-Assembly of Bombyx mori Silk Fibroin

            Na Kong

8. Synthesis and Assembly of Recombinant Collagen

            Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, and Jing Ren

9. Recombination and Purification of Elastin-Like Polypeptides

            Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, and Jing Ren

10. Methods to Synthesize and Assemble Recombinant Keratins

            Wenwen Zhang and Yimin Fan

11. Preparation of Amyloid Fibers Using Recombinant Technology

            Wenwen Zhang and Yimin Fan

12. Molecular Biology Methods to Construct Recombinant Fibrous Protein

            Jicong Zhang

Part III: Methods in Assembly of Fibrous Proteins

13. Isolation of Nanofibrils from Animal Silks

            Ke Zheng

14. Gelation Methods to Assemble Fibrous Proteins

            Ning Fan and Ke Zheng

15. Spinning Methods Used for The Construction of One- and Two-Dimensional Fibrous Protein Materials

            Leitao Cao

16. Three-Dimensional Printing to Build Fibrous Protein Architectures

            Huanhuan Qiao and Ke Zheng

Part IV: Methods in Characterization of Fibrous Proteins

17. The Methods of Using Synchrotron FTIR Microspectroscopy to Understand the Conformation of Single Animal Silk Fibers

            Chao Ye, Leitao Cao, and Shengjie Ling

18. The Protocol of Using FTIR Imaging to Investigate Silk Fibroin-Based Materials

            Jiajia Zhong, Xiaojie Zhou, Chao Ye, Wenjie Yu, and Yuzhao Tang

19. Secondary Structure Analysis of Single Silk Nanofibril through Infrared Nanospectroscopy

            Yawen Liu, Hongchong Guo, and Shengjie Ling

20. The Method of Using Raman Spectroscopy to Understand the Conformation of Fibrous Proteins

            Wenli Gao, Ting Shu, Qiang Liu, Hongchong Guo, Shengjie Ling, and Liang Zhou

21. Structural Characterization of Silk Fibers by Wide-Angle X-Ray Scattering

            Zhuochen Lv, Ping Qi, Leitao Cao, and Shengjie Ling

22. Structural Characterization of Silk Fibers by Small-Angle X-Ray Scattering

            Zhuochen Lv, Ping Qi, Leitao Cao, and Shengjie Ling

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts