What's Wrong With My Mouse? (2^nd Ed.) Behavioral Phenotyping of Transgenic and Knockout Mice

Turn to What?s Wrong with My Mouse? to discover the wealth of mouse behavioral tasks and to get the guidance you need to select the best methods and necessary controls. Chapters are organized by behavioral domain, including measurements of general health, motor functions, sensory abilities, learning and memory, feeding and drinking, reproductive, social, emotional, and reward behaviors in mutant mice. Throughout the chapters, new behavioral tasks and new research discoveries have been added, bringing the Second Edition up to date with the latest science. In addition, the Second Edition includes two new chapters:

"Neurodevelopment and Neurodegeneration" discusses mouse behavioral tasks relevant to neurodevelopmental diseases, such as mental retardation and autism, and to neurodegenerative diseases, such as Alzheimers, Parkinsons, Huntingtons, and amyotrophic lateral sclerosis.

"Putting It All Together" recommends strategies for optimizing a battery of behavioral phenotyping tests to address your specific hypotheses about gene functions.

The final chapter, "The Next Generation," examines new and emerging technologies.

Throughout the book, the use of behavioral testing equipment is illustrated with photographs, diagrams, and representative data. Examples of behavioral tasks successfully applied to transgenic and knockout mouse models are provided, as well as references to the primary literature and step-by-step methods protocols. These features, along with a comprehensive index, listings of database and vendor websites, and an extensive list of references, make this book a valuable and practical resource for students and researchers.

Preface ix

Preface to the First Edition xi

Acknowledgments xiii

Chapter 1 Designer Mice 1
Scope and sourcebooks

Chapter 2 Of Unicorns and Chimeras 10
How to generate a line of transgenic or knockout mouse for behavioural phenotyping

Chapter 3 General Health 42
Give your mouse a physical

Chapter 4 Motor Functions 62
Open field, holeboard, rotarod, balance, grip, circadian activity, circling, stereotypy, ataxic gait, seizures

Chapter 5 Sensory Abilities 86
Olfaction, vision, hearing, taste, touch, nociception

Chapter 6 Learning and Memory 110
Morris swim task, spatial mazes, cued and contextual conditioning, conditioned taste aversion, conditioned eyeblink, olfactory discrimination, social recognition, passive avoidance, schedule controlled operant tasks, motor learning, attention

Chapter 7 Feeding and Drinking 164
Daily consumption, restricted access, choice tests, microstructural analysis

Chapter 8 Reproductive Behaviors 186
Sexual and parental behaviors

Chapter 9 Social Behaviors 206
Social interaction, nesting, grooming, juvenile play, aggression

Chapter 10 Emotional Behaviors: Animal Models of Psychiatric Diseases 226
Mouse models of fear, anxiety, depression, schizophrenia

Chapter 11 Reward 266
Self-administration of addictive drugs, conditioned place preference

Chapter 12 Neurodevelopment and Neurodegeneration 290
Assaying behaviors in infant, juvenile, and aged mice

Chapter 13 Putting It All Together 322
Choice of tests, order of testing, number of mice, equipment, housing and testing environment

Chapter 14 The Next Generation 344
Conditional and inducible mutations, viral vector gene delivery, RNA silencing, quantitative trait loci analysis, DNA microarrays, chemical mutagenesis, gene therapy, ethical issues

References 383

Index 503

What's Wrong With My Mouse?: Behavioral Phenotyping of Transgenic and Knockout Mice, Second Edition has been written in Dr. Crawley's private capacity, outside of her professional position at the National Institutes of Health. The views expressed in this book do not necessarily represent the views of the National Institutes of Health or of the United States government.