Vaccine Adjuvants, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1494

Overview of Vaccine Adjuvants: Introduction, History, and Current Status.- Development of the CpG Adjuvant 1018: A Case Study.- Syntheses of Human TLR8-Specific Small-Molecule Agonists.- Semisynthesis of Analogues of the Saponin Immunoadjuvant QS-21.- QS-21 Adjuvant: Laboratory-Scale Purification Method and Formulation into Liposomes.- Purification of an Immunoadjuvant Saponin Fraction from Quillaja brasiliensis Leaves by Reverse-Phase Silica Gel Chromatography.- Biosynthetic Approaches to Squalene Production: The Case of Yeast.- In Silico Adjuvant Design and Validation.- Manufacturing Methods for Liposome Adjuvants.- Synthesis of Lymph Node-Targeting Adjuvants.- Preparing an Adjuvanted Thermoresponsive Gel Formulation for Sublingual Vaccination.- Manufacture of Oil-in-Water Emulsion Adjuvants.- Methods to Prepare Aluminum Salt-Adjuvanted Vaccines.- Production of Adjuvant-Loaded Biodegradable Particles for Use in Cancer Vaccines.- Lyophilization of Adjuvanted Vaccines: Methods for Formulation of a Thermostable Freeze Dried Product.- Stressed Stability Techniques for Adjuvant Formulations.- Particle Sizing of Nanoparticle Adjuvant Formulations by Dynamic Light Scattering (DLS) and Nanoparticle Tracking Analysis (NTA).- Quantification of Multiple Components of Complex Aluminum-Based Adjuvant Mixtures by Using Fourier Transform Infrared Spectroscopy and Partial Least Squares Modeling.- Determination of Protein Content in Alhydrogelâ-Based Vaccines by O-Phthalaldehyde Assay.- Staining and Transfer Techniques for SDS-PAGE Gels to Minimize Oil-in-Water Emulsion Adjuvant Interference.- Interactions Between Antigens and Nanoemulsion Adjuvants: Separation and Characterization Techniques.- Screening Vaccine Formulations in Fresh Human Whole Blood.- Analysis of the Innate Response to Adjuvants: Characterization of the Draining Lymph Node by Fluorescence-Activated Cell Sorting.- Assessment of Antigen-Specific Cellular Immunogenicity Using Intracellular Cytokine Staining, ELISpot, and Culture Supernatants.- Eliciting Epitope-Specific CD8 T Cell Response by Immunization with Microbial Protein Antigens Formulated with α-Galactosylceramide: Theory, Practice, and Protocols.- Molecular Methods and Bioinformatic Tools for Adjuvant Characterization by High-Throughput Sequencing.