TRP Channels in Drug Discovery, 2012 Volume I Methods in Pharmacology and Toxicology Series

Recent findings have implied a distinct therapeutic potential for drugs targeting Transient Receptor Potential (TRP) channels in a wide variety of diseases, many with no existing satisfactory treatment options. Thus, the TRP superfamily of ion channels has attracted a great deal of well-deserved attention.  TRP Channels in Drug Discovery provides a thorough collection of the most up-to-date reviews and protocols on the subject, coming from top experts in the field.  Volume I presents a series of state-of-the-art minireviews on the most interesting TRP channels (from TRPA1 to TRPV4), as well as TRP-related protocols involving airways and the genitourinary tract.  Written for the Methods in Pharmacology and Toxicology? series, this work includes the kind of detailed description and key implementation advice that ensures successful results in the lab.

Part I: Introduction

1. Introduction to TRPs: A Quest for Novel Drug Targets

            Bernd Nilius

2. TRP-Mediated Cytoskeletal Reorganization: Implications for Disease and Drug Development

            Chandan Goswami

Part II: TRP Channels, from A1 to V4

3. TRPA1 in Drug Discovery

            Jun Chen, Steve McGaraughty, and Philip R. Kym

4. Canonical Transient Receptor Potential Channel (TRPC) Expression, Regulation, and Function in Vascular and Airway Diseases

            Brij B. Singh, Christina M. Pabelick, and Y.S. Prakash

5. TRPM2 Function and Potential as a Drug Target

            Barbara A. Miller

6. The Ca²⁺-Activated Monovalent Cation Selective Channels TRPM4 and TRPM5

            Colsoul Barbara, Kecskes Miklos, Philippaert Koenraad, Menigoz Aurelie, and Vennekens Rudi

7. The Emerging Role of TRPM7 in the Regulation of Magnesium Homeostasis

            Vladimir Chubanov, Jonathan T. Eggenschwiler, Thomas Gudermann, and Alexey G. Ryazanov

8. TRPM8 Channels as Potential Therapeutic Targets for Pain, Analgesia, and Thermoregulation

            David D. McKemy

9. TRPML Channels in Function, Disease, and Prospective Therapies

            David A. Zeevi

10. The TRPP Signalling Module: TRPP2/Polycystin 1 and TRPP2/PKD1L1

            Alexis Hofherr

11. TRPV1 as a Polymodal Sensor: Potential to Discover TRPV1 Antagonists Selective for Specific Activating Modalities

            Olivier Radresa, Stephen Zicha, William Brown, and Jennifer M.A. Laird

12. Nociceptive and Non-Nociceptive Roles of TRPV3 and Its “Druggability”

            Sungjae Yoo and Sun Wook Hwang

13. TRPV4 and Drug Discovery

            Fabien Vincent and Matthew A.J.  Duncton

Part III: TRPs and Airways

14. TRP Expression and Function in the Lung Airways

            Alexander Dietrich and Thomas Gudermann

15. Hypoxia-Dependent TRP Channel Function in Pulmonary Arterial Smooth Muscle Cells

            Beate Fuchs, Hermann Kalwa, Norbert Weissmann, Thomas Gudermann, and Alexander Dietrich

16. Endotoxin-Induced Airway Inflammation and Asthma Models

            Zsuzsanna Helyes and Zsófia Hajna

17. Enhanced Cough, Animal Models

            Raffaele Gatti, Pamela Pedretti, Romina Nassini, and Marcello Trevisani

18. Capsaicin Inhalation Test in Man

            Eva Millqvist

Part IV: TRPs and the Genitourinary (GU) Tract

19. TRP Channels in the Genitourinary (GU) Tract

            Ana Charrua and Francisco Cruz

20. Animal Models of Cystitis

            Célia D. Cruz and António Avelino

21. Overactive Bladder Models

            Roberto Soler, Lysanne Campeau, Claudius Füllhase, and Karl-Erik Andersson

            Alexis Hofherr

11. TRPV1 as a Polymodal Sensor: Potential to Discover TRPV1 Antagonists Selective for Specific Activating Modalities

            Olivier Radresa, Stephen Zicha, William Brown, and Jennifer M.A. Laird

12. Nociceptive and Non-Nociceptive Roles of TRPV3 and Its “Druggability”

            Sungjae Yoo and Sun Wook Hwang

13. TRPV4 and Drug Discovery

            Fabien Vincent and Matthew A.J.  Duncton

Part III: TRPs and Airways

14. TRP Expression and Function in the Lung Airways

            Alexander Dietrich and Thomas Gudermann

15. Hypoxia-Dependent TRP Channel Function in Pulmonary Arterial Smooth Muscle Cells

            Beate Fuchs, Hermann Kalwa, Norbert Weissmann, Thomas Gudermann, and Alexander Dietrich

16. Endotoxin-Induced Airway Inflammation and Asthma Models

            Zsuzsanna Helyes and Zsófia Hajna

17. Enhanced Cough, Animal Models

            Raffaele Gatti, Pamela Pedretti, Romina Nassini, and Marcello Trevisani

18. Capsaicin Inhalation Test in Man

            Eva Millqvist

Part IV: TRPs and the Genitourinary (GU) Tract

19. TRP Channels in the Genitourinary (GU) Tract

            Ana Charrua and Francisco Cruz

20. Animal Models of Cystitis

            Célia D. Cruz and António Avelino

21. Overactive Bladder Models

            Roberto Soler, Lysanne Campeau, Claudius Füllhase, and Karl-Erik Andersson

            Olivier Radresa, Stephen Zicha, William Brown, and Jennifer M.A. Laird

12. Nociceptive and Non-Nociceptive Roles of TRPV3 and Its “Druggability”

            Sungjae Yoo and Sun Wook Hwang

13. TRPV4 and Drug Discovery

            Fabien Vincent and Matthew A.J.  Duncton

Part III: TRPs and Airways

14. TRP Expression and Function in the Lung Airways

            Alexander Dietrich and Thomas Gudermann

15. Hypoxia-Dependent TRP Channel Function in Pulmonary Arterial Smooth Muscle Cells

            Beate Fuchs, Hermann Kalwa, Norbert Weissmann, Thomas Gudermann, and Alexander Dietrich

16. Endotoxin-Induced Airway Inflammation and Asthma Models

            Zsuzsanna Helyes and Zsófia Hajna

17. Enhanced Cough, Animal Models

            Raffaele Gatti, Pamela Pedretti, Romina Nassini, and Marcello Trevisani

18. Capsaicin Inhalation Test in Man

            Eva Millqvist

Part IV: TRPs and the Genitourinary (GU) Tract

19. TRP Channels in the Genitourinary (GU) Tract

            Ana Charrua and Francisco Cruz

20. Animal Models of Cystitis

            Célia D. Cruz and António Avelino

21. Overactive Bladder Models

            Roberto Soler, Lysanne Campeau, Claudius Füllhase, and Karl-Erik Andersson

            Sungjae Yoo and Sun Wook Hwang

13. TRPV4 and Drug Discovery

            Fabien Vincent and Matthew A.J.  Duncton

Part III: TRPs and Airways

14. TRP Expression and Function in the Lung Airways

            Alexander Dietrich and Thomas Gudermann

15. Hypoxia-Dependent TRP Channel Function in Pulmonary Arterial Smooth Muscle Cells

            Beate Fuchs, Hermann Kalwa, Norbert Weissmann, Thomas Gudermann, and Alexander Dietrich

16. Endotoxin-Induced Airway Inflammation and Asthma Models

            Zsuzsanna Helyes and Zsófia Hajna

17. Enhanced Cough, Animal Models

            Raffaele Gatti, Pamela Pedretti, Romina Nassini, and Marcello Trevisani

18. Capsaicin Inhalation Test in Man

            Eva Millqvist

Part IV: TRPs and the Genitourinary (GU) Tract

19. TRP Channels in the Genitourinary (GU) Tract

            Ana Charrua and Francisco Cruz

20. Animal Models of Cystitis

            Célia D. Cruz and António Avelino

21. Overactive Bladder Models

            Roberto Soler, Lysanne Campeau, Claudius Füllhase, and Karl-Erik Andersson

            Fabien Vincent and Matthew A.J.  Duncton

Part III: TRPs and Airways

14. TRP Expression and Function in the Lung Airways

            Alexander Dietrich and Thomas Gudermann

15. Hypoxia-Dependent TRP Channel Function in Pulmonary Arterial Smooth Muscle Cells

            Beate Fuchs, Hermann Kalwa, Norbert Weissmann, Thomas Gudermann, and Alexander Dietrich

16. Endotoxin-Induced Airway Inflammation and Asthma Models

            Zsuzsanna Helyes and Zsófia Hajna

17. Enhanced Cough, Animal Models

            Raffaele Gatti, Pamela Pedretti, Romina Nassini, and Marcello Trevisani

18. Capsaicin Inhalation Test in Man

            Eva Millqvist

Part IV: TRPs and the Genitourinary (GU) Tract

19. TRP Channels in the Genitourinary (GU) Tract

            Ana Charrua and Francisco Cruz

20. Animal Models of Cystitis

            Célia D. Cruz and António Avelino

21. Overactive Bladder Models

            Roberto Soler, Lysanne Campeau, Claudius Füllhase, and Karl-Erik Andersson

            Raffaele Gatti, Pamela Pedretti, Romina Nassini, and Marcello Trevisani

18. Capsaicin Inhalation Test in Man

            Eva Millqvist

Part IV: TRPs and the Genitourinary (GU) Tract

19. TRP Channels in the Genitourinary (GU) Tract

            Ana Charrua and Francisco Cruz

20. Animal Models of Cystitis

            Célia D. Cruz and António Avelino

21. Overactive Bladder Models

            Roberto Soler, Lysanne Campeau, Claudius Füllhase, and Karl-Erik Andersson

            Eva Millqvist

Part IV: TRPs and the Genitourinary (GU) Tract

19. TRP Channels in the Genitourinary (GU) Tract

            Ana Charrua and Francisco Cruz

20. Animal Models of Cystitis

            Célia D. Cruz and António Avelino

21. Overactive Bladder Models

            Roberto Soler, Lysanne Campeau, Claudius Füllhase, and Karl-Erik Andersson

19. TRP Channels in the Genitourinary (GU) Tract

            Ana Charrua and Francisco Cruz

20. Animal Models of Cystitis

            Célia D. Cruz and António Avelino

21. Overactive Bladder Models

            Roberto Soler, Lysanne Campeau, Claudius Füllhase, and Karl-Erik Andersson

            Ana Charrua and Francisco Cruz

20. Animal Models of Cystitis

            Célia D. Cruz and António Avelino

21. Overactive Bladder Models

            Roberto Soler, Lysanne Campeau, Claudius Füllhase, and Karl-Erik Andersson

            Roberto Soler, Lysanne Campeau, Claudius Füllhase, and Karl-Erik Andersson

Features reviews of the most interesting TRP channels, from TRPA1 to TRPV4

Includes clear and focused protocols on TRP channels and disorders of airways and the GU tract

Contains important implementation tips for the lab-ready, tested protocols

Includes supplementary material: sn.pub/extras

Includes supplementary material: sn.pub/extras