Synthetic Gene Circuits , 1st ed. 2021 Methods and Protocols Methods in Molecular Biology Series, Vol. 2229

This volume provides clear and direct protocols to implement automated Design-Build-Test-Learn (DBTL) into synthetic biology research. Chapters detail techniques to model and simulate biological systems, redesign biological systems, setting up of an automated biolaboratory, step-by-step guide on how to perform computer aided design, RNA sequencing, microfluidics -using bacterial cell free extracts, live mammalian cells, computational and experimental procedures, metabolic burden, computational techniques to predict such burden from models, and how DNA parts can be engineered in mammalian cells to sense, and respond to, and intracellular signals in general. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

  Authoritative and cutting-edge,  Synthetic Gene Circuits: Methods and Protocols aims to ensure successful results in the further study of this vital field.

1. Qualitative Modeling, Analysis and Control of Synthetic Regulatory Circuits

Madalena Chaves and Hidde de Jong

Jesus Pico, Alejandro Vignoni, and Yadira Boada

3. Using Models To (Re-)Design Synthetic Circuits

Giselle McCallumand Laurent Potvin-Trottier

4. Automated bio-circuit design with SYNBADm

5. Setting up an Automated Biomanufacturing Laboratory

Marilene Pavan

Valentin Zulkower

Valentin Zulkower

8. Characterizing Genetic Parts and Devices using RNA Sequencing

Deepti Vipin, Zoya Ignatova, and Thomas E. Gorochowski

9. Steady-State Cell-Free Gene Expression with Microfluidic Chemostats

10. A Microfluidic/Microscopy-Based Platform for On-Chip Controlled Gene Expression in Mammalian Cells

Mahmoud Khazim, Elisa Pedone, Lorena Postiglione, Diego di Bernardo, and Lucia Marucci

11. Optimal Experimental Design for Systems and Synthetic Biology Using Amigo2

Eva Balsa-Canto, Lucia Bandiera, and Filippo Menolaschina

12. A Cyber-Physical Platform for Model Calibration

Lucia Bandiera, David Gomez Cabeza, Eva Balsa-Canto, and Filippo Menolascina

13. Prediction of cellular burden with host-circuit models

Evangelos-Marios Nikolados, Andrea Y. Weiße, and Diego A. Oyarz´un

Andras Gyorgy

15. Engineering Sensors for Gene Expression Burden

16. Engineering Protein-based Parts for Genetic Devices in Mammalian Cells

Giuliano Bonfa, Federica Cella, and Velia Siciliano

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts