Steroid Receptors, Softcover reprint of the original 1st ed. 2014 Methods and Protocols Methods in Molecular Biology Series, Vol. 1204

Steroid Receptors: Methods and Protocols presents a selection of techniques that have been recently applied to the analysis of steroid receptors, powerful tools for the advancement of our understanding of both the mechanisms regulating gene transcription and the rapid signaling responses of tissues to signals. Research in this area has generated a wealth of data allowing the elucidation of steroid receptor mechanisms and improving the treatment of many endocrine disorders, above all cancers. Chapters cover methods to analyze gene transcription, chromatin and proteomic modifications, extra-nuclear signaling regulation, development of cell and animal models, and preparation of new antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Steroid Receptors: Methods and Protocols offers an updated view on a variety of modern methods that will hopefully contribute to improving our knowledge on the integration of steroid receptors in single or different functionally connected cellular contexts under a variety of physiological and pathological conditions, above all tumors.

Progesterone Receptor Interaction with Chromatin.- Mapping the Genomic Binding Sites of the Activated Retinoid X Receptor in Murine Bone Marrow-Derived Macrophages using Chromatin Immunoprecipitation Sequencing.- Analysis of Chromatin-Nuclear Receptor Interactions by Laser-Chromatin Immunoprecipitation.- Examining Estrogen Regulation of Cancer Stem Cells through Multicolor Lineage Tracing.- Reporter Mice for the Study of Long Term Effects of Drugs and Toxic Compounds.- Analysis of Histone post-Translational Modifications in the Control of Chromatin Plasticity Observed at Estrogen-Responsive Sites in Human Breast Cancer Cells.- In Silico Analysis of Genomic Data for Construction of Nuclear Receptor Network.- Cofactor Profiling of the Glucocorticoid Receptor from a Cellular Environment.- Paxillin and Steroid Signaling: from Frog to Human.- Analysis of the Androgen Receptor/Filamin A Complex in Stromal Cells.- Multi-Well Plate Immunoassays for Measuring Signaling Protein Activations/Deactivations and Membrane Vs. Intracellular Receptor Levels.- Promixity Ligation Assay to Detect and Localize the Interactions of ERa with PI3-K and Src in Breast Cancer Cells and Tumor Samples.- Phosphoinositide 3-Kinase Assay in Breast Cancer Cell Extracts.- Rapid Estrogen Effects on Aromatase Phosphorylation in Breast Cancer Cells.- Mouse Monoclonal Antibodies against Estrogen Receptor.- Analysis of Estrogen Receptor Interacting Proteins Using Pull Down Assay and MALDI-MS Methods.- Analysis of the Conformation of the Androgen Receptor in Spinal Bulbar Muscular Atrophy by Atomic Force Microscopy.- Imaging of Corticosteroid Receptors in Live Cells.- Physiological Techniques in the Study of Rapid Aldosterone Effects.- Detection of the Glucocorticoid Receptors in Brain Protein Extracts by SDS-PAGE.