Single-Molecule Metal-Induced Energy Transfer, 1st ed. 2017 From Basics to Applications Springer Theses Series

This thesis presents a novel single-molecule spectroscopy method that, for the first time, allows the dipole orientations and fluorescence lifetimes of individual molecules to be measured simultaneously. These two parameters are needed to determine the position of individual molecules with nanometer accuracy near a metallic structure. Proof-of-principle experiments demonstrating the value of this new single-molecule localization concept are also presented. Lastly, the book highlights potential applications of the method in biophysics, molecular physics, soft matter and structural biology.

Nominated as an outstanding PhD thesis by Göttingen University, Germany

Introduces the electrodynamics of a dipole emitter from fundamentals with illustrations

Includes descriptions of the optical setups used, together with possible modifications/combinations that will be especially useful for experimental physicists

Features discussion/comments on the practical aspects of the method, highlighting its potential applications in biophysics, structural biology, and molecular physics

Includes supplementary material: sn.pub/extras