RNA Scaffolds, Softcover reprint of the original 1st ed. 2015 Methods and Protocols Methods in Molecular Biology Series, Vol. 1316

Practical and authoritative, RNA Scaffolds: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.

1. A Method to Predict the 3D Structure of an RNA Scaffold

Xiaojun Xu and Shi-Jie Chen

2. Post-crystallization Improvement of RNA Crystal Diffraction Quality

Jinwei Zhang and Adrian R. Ferré-D’Amaré

3. Expression and Purification of RNA-protein Complexes in Escherichia coli 

El Khouri M, Catala M, Seijo B, Chabal J, Tisné C, Dardel F, and  Ponchon L

4. Production of Homogeneous Recombinant RNA using a tRNA Scaffold and Hammerhead Ribozymes

Frank H.T. Nelissen, Hans A. Heus, and Sybren S. Wijmenga

5. In vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold

Victor G. Stepanov and George E. Fox

6. Detection of RNA-Protein Interactions using Tethered RNA Affinity Capture

IIOKA H. and Macara I.G.

7. A Universal Method for Labeling Native RNA in Live Bacterial Cells

Irina Smolina and Natalia Broude

8. Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors

Colleen A. Kellenberger, Zachary F. Hallberg, and Ming C. Hammond

9. RNA Scaffold: Designed To Co-Localize Enzymes

ZJU_China team and Ming Chen

10. Artificial Ligase Ribozymes Isolated by a “Design And Selection” Strategy

Shigeyoshi Matsumura and Yoshiya Ikawa

11. Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach

Charlotte Rehm, Benedikt Klauser, and Jörg S. Hartig

12. Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells

Yoko Nomura and Yohei Yokobayashi

13. Design and Characterisation of Topological Small RNAs

Jack Hassall, Paul MacDonald, Teresa Cordero, William Rostain, and Alfonso Jaramillo

14. Folding RNA-Protein Complex into Designed Nanostructures

Tomonori Shibata, Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo, and Hirohide Saito

15. Simple Method for Constructing RNA Triangle, Square, Pentagon by Tuning Interior RNA 3WJ Angle from 60˚ to 90˚ or 108˚

Emil F. Khisamutdinov, My Nguyen Hoan Bui, Daniel Jasinski, Zhengyi Zhao, Zheng Cui, and Peixuan Guo

16. RNA-Mediated CdS Based Nanostructures

Vinit Kumar and Anil Kumar

17. An Effective Method for Specific Gene Silencing in Escherichia coli using Artificial Small RNA

Geunu Bak, Jee Soo Choi, Wonkyeong Kim, Shinae Suk, and Younghoon Lee

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts