Research Methods in Biomineralization Science

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in biomineralization science, and includes sections on such topics as determining solution chemistry, structure and nucleation; probing structure and dynamics at surfaces; and interfaces mapping biomineral and morphology and ultrastructure.

Determining solution chemistry, structure and nucleation 1. Development of accurate force fields for the simulation of biomineralisation 2. The integration of ion potentiometric measurements with chemical, structural and morphological analysis to follow mineralization reactions in solution. 3. Investigating the Early Stages of Mineral Precipitation by Potentiometric Titration and Analytical Ultracentrifugation 4. Replica Exchange Methods in Biomineral Simulations 5. SAXS in inorganic and bio-inspired research 6. In situ Solution Study of Calcium Phosphate Crystallization Kinetics

Probing structure and dynamics at surfaces and interfaces 7. Design, fabrication, and applications of in situ fluid cell TEM to mineralization reactions 8. X-ray Absorption Spectroscopy for the Structural Investigation of Self-Assembled-Monolayer-Directed Mineralization 9. Cryo-TEM Analysis of Collagen Fibrillar Stucture

Biomimetic crystallization techniques in vitro and in silico 10. Preparation of organothiol self-assembled monolayers for use in templated crystallization 11. Experimental techniques for the growth and characterization of silica biomorphs and silica gardens 12. Precipitation in liposomes as a model for intracellular biomineralization 13. Polymer Mediated Growth of Crystals and Mesocrystals 14. Phage display for the discovery of hydroxyapatite associated peptides

Protein structure, interactions and function 15. Quantitatively and Kinetically Identifying Binding Motifs of Amelogenin Proteins to Mineral Crystals through Biochemical and Spectroscopic Assays 16. Using the RosettaSurface Algorithm to Predict Protein Structure at Mineral Surfaces 17. Investigating Protein Function in Biomineralized Tissues using Molecular Biology Techniques

Mapping biomineral morphology and ultrastructure 18. Imaging the nanostructure of bone and dentin through small- and wide-angle scattering 19. Synchrotron X-ray Nanomechanical Imaging of Mineralized Fibre Composites

Mapping mineral chemistry 20. Application of Total X-ray Scattering Methods and Pair Distribution Function Analysis for Study of Structure of Biominerals 21. X-ray Microdiffraction of Biominerals 22. FT-IR and Raman studies of structure and bonding in mineral and organic-mineral composites 23. A mixed flow reactor method to synthesize amorphous calcium carbonate under controlled chemical conditions

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

• Continues the legacy of this premier serial with quality chapters authored by leaders in the field
• Covers research methods in biomineralization science
• Contains sections on such topics as and includes sections on such topics as determining solution chemistry, structure and nucleation; probing structure and dynamics at surfaces; and interfaces mapping biomineral and morphology and ultrastructure