Reporter Genes, 2007 A Practical Guide Methods in Molecular Biology Series, Vol. 411

Langue : Anglais

Coordonnateur : Anson Don

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Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. This book describes practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available and will concentrate on those marker genes that are currently most commonly used.

Methodologies for Staining and Visualisation of ?-Galactosidase in Mouse Embryos and Tissues.- Immunohistochemical Detection of ?-Galactosidase or Green Fluorescent Protein on Tissue Sections.- Detection of Reporter Gene Expression in Murine Airways.- Three-Dimensional Analysis of Molecular Signals with Episcopic Imaging Techniques.- Fluorescent Proteins for Cell Biology.- Detection of GFP During Nervous System Development in Drosophila melanogaster.- Autofluorescent Proteins for Flow Cytometry.- Fluorescent Protein Reporter Systems for Single-Cell Measurements.- Subcellular Imaging of Cancer Cells in Live Mice.- Noninvasive Imaging of Molecular Events with Bioluminescent Reporter Genes in Living Subjects.- Green Fluorescent Protein as a Tracer in Chimeric Tissues.

Provides practical guides for the use of the reporter genes most commonly used in mammalian systems including b-galactosidase, GFP and luciferase Easy to follow protocols for the use of reporter genes in various eukaryotic systems