Protein Discovery Technologies

A True Insider?s Guide to the Field ? Then and Now

Until now, there has not been a book that effectively addresses the historical basis of protein discovery. Featuring contributions from a distinguished international panel of experts, Protein Discovery Technologies elucidates the principles, techniques, strategies, and broad range of applications of protein discovery by documenting the often untold stories and personal accounts of the contributors? past scientific achievements.

Incorporates Short, Personal Discovery Vignettes

Rather than an exhaustive field analysis, this globally pertinent resource presents in-depth discussions of various methods for protein discovery, including bioinformatics, interaction cloning, protein purification, phage display, non-primate models, and chemical targeting. It also explores biological themes through the examination of cell death, angiogenesis, hemostasis, development, signal transduction, transcriptional control, cell cycle control, neurobiology, and quality control.

This readily accessible resource discusses an array of interesting topics, including:

• Tumor necrosis factors



• The origin of interferon as an angiogenesis inhibitor



• In vivo combinatorial mapping of vascular zip codes



• Extracellular matrix degradome as regulators of angiogenesis and tumor growth



• A matricellular protein prototype



• The use of RING finger proteins as E3 Ubiquitin Ligases

Whereas most books tend to be more specialized, this book contains a broad view of the protein discoveries in many different fields, making Protein Discovery Technologies a valuable reference for today?s researchers, both the new and more seasoned, who are seeking a newfound perspective or a deeper understanding of this exciting field.

Discovery of Vascular Permeability Factor (VPF, VEGF, VPF/VEGF, VEGF-A165). Tumor Necrosis Factor and Its Family Members. Blood–Brain Barrier Models for Investigating CNS Pathologies. Identification of Activation of Latent TGFß as Principal In Vivo Function of Integrin avß6. Purification of Basic Fibroblast Growth Factor by Heparin Affinity. How It Came to Pass that Interferon Was Used as an Angiogenesis Inhibitor. Use of Phage Display to Discover Inhibitors of Cell Migration. Combinatorial Mapping of Vascular Zip Codes by In Vivo Phage Display. Chasing Elusive Cellular Prion Protein Receptor. Discovery of Molecular Chaperone GRP78 and GRP94 Inducible by Endoplasmic Reticulum Stress. Origin of Sphingomyelin Pathway. Endotoxin-Induced Myocarditis: A New Pathophysiological Entity in Systemic Inflammatory Response Syndrome. Discovery of DOCK180 Superfamily of Exchange Factors. Discovery of Extracellular Matrix Degradome as Novel Endogenous Regulators of Angiogenesis and Tumor Growth. RING Finger Proteins as E3 Ubiquitin Ligases. Discovery of Chemical Nature of Cross-Links in Collagen: A Personal Retrospective. Discovery of SPARC as Prototype for Matricellular Proteins.

Renata Pasqualini, Ph.D., is the Buchanan & Seeger Professor of Medicine and Cancer Biology at The University of Texas M. D. Anderson Cancer Center. She is an internationally recognized expert in vascular biology, metastasis, and angiogenesis. Dr. Pasqualini originally co-developed in vivo phage display, which enables combinatorial mapping of tissue and disease-specific molecular addresses in vivo, allowing for the development of ligand-directed targeted delivery of therapeutic and imaging agents.

Wadih Arap, M.D., Ph.D., is the Stringer Professor of Medicine and Cancer Biology and serves as an Attending Physician and Deputy Chairman of the Department of Genitourinary Medical Oncology at The University of Texas M. D. Anderson Cancer Center. He is also a member of the Board of Scientific Counselors of the National Cancer Institute.