Principles & Practice of Assisted Reproductive Technology (3 Vols)

This three volume set is a comprehensive guide to Assisted Reproductive Technology (ART) for clinicians.

Volume one begins with an introduction to infertility, describing physiology, endocrinology and infertility in both men and women. The following sections provide in depth discussion on ART, from ovulation induction and intrauterine insemination, to complications, outcomes and ethical issues.

The second volume is dedicated to In Vitro Fertilisation (IVF) and related procedures, whilst volume three is an atlas of embryology.

This practical manual is an invaluable reference for clinicians specialising in infertility management and includes nearly 1000 full colour photographs, each with a brief description to enhance understanding.

Key points

• Three volume set – complete guide to ART
• Each volume dedicated to specific topic – Infertility, IVF & Related Procedures, and Atlas of Embryology
• Includes nearly 1000 photographs with descriptions
• Invaluable reference for practising clinicians

Volume 1: Infertility
SECTION 1: PHYSIOLOGY

• Anatomy and physiology of male & female reproductive system
• Ooogenesis and Folliculogenesis
• Physiology of Menstrual Cycle
• Human spermatozoa – Production and Migration
• The Human Sperm : Its Contribution to Fertilization and embryogenesis
• Endometrial receptivity
• Embryo maternal dialogue and Implantation

SECTION 2: REPRODUCTIVE ENDOCRINOLOGY

• Hyperprolactinemia and Thyroid disorders
• Hirsutism
• Fertility treatment in Primary and Secondary Amenorrhoea
• Luteal Phase defect
• Anovulatory infertility

SECTION 3: MALE INFERTILITY

• Etiology and Pathophysiology of male infertility
• Clinical and Endocrinological evaluation of the infertile male
• The Microbiology of semen and Infections of the Male Genito urinary tract
• Sexual Dysfunction
• Cytogenetics of male infertility
• Role of sonography and doppler in male infertility
• Varicocele and its role in male infertility
• Medical Management of male infertility
• Management of Azoospermia
• Sperm Retrieval techniques

SECTION 4: GENERAL OVERVIEW

• Patient selection and management
• Immunology in Infertility
• Environmental factors affecting fertility
• Declining fertility

SECTION 5: FEMALE FACTOR INFERTILITY

• Evaluation of infertile female
• Uterine and Cervical factors in Infertility
• Tubal factors in Infertility
• Role of HSG and SSG in evaluating the fallopian tubes
• Infections and infertility
• Tuberculosis and Infertility
• Transvaginal sonography and Doppler in female Infertility
• Endoscopy in Infertility
• Assessment of ovarian reserve
• Endometriosis and Infertility
• Role of fibroids in infertility
• Reconstructive Surgeries enhancing infertility
• Obesity and Infertility
• Unexplained Infertility
• Fertility preservation in female patients

SECTION 6: OVULATION INDUCTION

• Drugs for Controlled ovarian stimulation
• Super Ovulation Strategies in Assisted conception
• GnRH Agonists
• GnRH Antagonists
• Monitoring of ovulation induction
• Ovulation trigger
• Individualized stimulation protocol
• Mild stimulation protocols
• Role of LH in ovarian stimulation
• Progesterone in ovarian stimulation
• Prognosticating and Optimizing IVF outcome
• Luteal phase support

SECTION 7: INTRAUTERINE INSEMINATION

• Intrauterine Insemination
• Optimizing success in IUI

SECTION 8: ASSISTED REPRODUCTIVE TECHNIQUES

• Anaesthesia and IVF
• Oocyte retrieval
• Embryo Transfer

SECTION 9: DILEMMAS IN ART

• Treatment strategies in poor responders
• Recurrent Implantation Failure
• Empty Follicle Syndrome
• Single Embryo Transfer
• Role of Aneuploidy screening in pre implantation embryos
• Epigenetics and AR

SECTION 10: COMPLICATIONS OF ART

• Ovarian Hyperstimulation Syndrome
• Ectopic Pregnancy
• Latrogenic multiple pregnancy
• Fetal Reduction
• Oncogenic risks related to ART

SECTION 11: THIRD PARTY REPRODUCTION

• Oocyte and Embryo Donation
• Oocyte Sharing Programmes
• Surrogacy
• Challenges faced in setting up a Surrogate house within the ICMR guidelines
• Adoption

SECTION 12: MISCELLANEOUS

• Batch IVF
• Alternatives of ART
• Endometrial preparation in FET cycles

SECTION 13: OUTCOME FOLLOWING ART

• Obstetric outcome following ART
• Outcome of children born through ART

SECTION 14: GRIEF COUNSELLING

SECTION 15: ETHICAL AND LEGAL ISSUES

• Ethical legal and social issues of modern ART treatment

SECTION 16: FUTURE CLINICAL APPLICATIONS

• Endometrial stem cells and reproduction

Volume 2: IVF and Related Procedures

SECTION 1: ANDROLOGY

• Semen Analysis in 21^st Century Medicine—A Paradigm Shift
• Sperm Chromatin Assessment
• Ejaculatory Failure
• Sperm Preparation in Assisted Conception: Laboratory Techniques
• Semen Banking

SECTION 2: EMBRYOLOGY

• History of In Vitro Fertilization
• In Vitro Fertilization and Embryo Transfer
• Intracytoplasmic Sperm Injection
• Analysis of Fertilization
• Morphological Assessment of Oocyte and Embryo
• Blastocyst Culture and Transfer 
• Advantages and Disadvantages of Embryo Transfer on Day 2, Day 3 and Blastocyst Stage
• Embryo Transfer Techniques
• Cryopreservation of Oocytes and Embryos by Slow Freezing Method
• Oocyte Vitrification
• Vitrification

SECTION 3: ADVANCES IN ASSISTED REPRODUCTVE TECHNOLOGIES

• Assisted Hatching
• Embryo Biopsy for Preimplantation Genetic Diagnosis
• In Vitro Maturation of Oocytes: A Practical Approach
• Ovarian Tissue Cryopreservation
• Time Lapse Video for Assessing the Embryos
• Polarization Microscopy
• OMICS
• IMSI and its Clinical Significance
• Microfluidics in ART

SECTION 4: ETHICAL AND LEGAL ISSUES

• Certification and Accreditation-Relevance to an IVF Center
• Accreditation of an ART Laboratory

SECTION 5: LABORATORY MANAGEMENT

• Setting-up an ART Laboratory
• Quality Control of IVF Laboratory
• Culture Systems
• Micromanipulators
• Troubleshooting in ART

Volume 3: Atlas of Embryology

• Oocyte Cumulus Complex Evaluation
• Oocyte Nuclear Maturity Evaluation
• Oocyte Dysmorphisms
• The Sperm
• Normal Embryo Development
• Embryo Scoring
• In Vitro Fertilization/Intracytoplasmic Sperm Injection
• Advanced Techniques
• Cryopreservation
• Assisted Reproductive Technology Lab Setting

Kamini Rao DGO DORCP DCh FRCOG (UK) MCh (UK) FICOG PGDMLE (Law)
Milann Infertility Centre, Jayanagar, Bangalore, Karnataka, India