Plant Organogenesis, Softcover reprint of the original 1st ed. 2013 Methods and Protocols Methods in Molecular Biology Series, Vol. 959

Organogenesis entails the regulation of cell division, cell expansion, cell and tissue type differentiation, and patterning of the organ as a whole. It is essential to gain insight into how organs are initiated and how they develop. However, this very often is subject to technical difficulties as these processes take place embedded deep in tissues or are difficult to access or visualize. To achieve this, we need specialized techniques such as those concisely illustrated in Plant Organogenesis: Methods and Protocols. Chapters address topics such as how to study and image the structure of ovules and embryos of Arabidopsis thaliana, tools to establish cell lineages in order to visualize the contribution of each cell and cell division to the building of a mature organ, approaches to study the totipotency of several plant cells, techniques such as the use of fluorescence-activated cell sorting (FACS) to analyse transcriptomes and hormone levels in Arabidopsis, methods to investigate organogenesis in economically important crops, and computer-based approaches to bring everything together. Written in the successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Plant Organogenesis: Methods and Protocols serves both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this fascinating researchfield.

The Tomato Leaf as a Model System for Organogenesis.- Usefulness of Physcomitrella patens for Studying Plant Organogenesis.- The Dicot Root as a Model System for Studying Organogenesis.- Genetic Control of Root Organogenesis in Cereals.- Gene Expression Analysis of Aquatic Angiosperms Podostemaceae to Gain Insight into the Evolution of Their Enigmatic Morphology.- Brown Algae as a Model for Plant Organogenesis.- Microscopic Analysis of Ovule Development in Arabidopsis thaliana.- Imaging of Phenotypes, Gene Expression and Protein Localization During Embryonic Root Formation in Arabidopsis.- Inducible System for Lateral Roots in Arabidopsis thaliana and Maize.- Adventitious Root Induction in Arabidopsis thaliana as a Model for in vitro Root Organogenesis.- High-Throughput, Non-Invasive Imaging of Root Systems.- Small Molecule Screens to Study Lateral Root Development.- Cell Lineage Analyses in Living Tissues.- Protein Immunolocalization in Maize Tissues.- Auxin Immunolocalization in Plant Tissues.- Gene Expression Analysis of Shoot Apical Meristem Cell Types.- Kinematic Analysis of Cell Division in Leaves of Mono- and Dicotyledonous Species: A Basis for Understanding Growth and Developing Refined Molecular Sampling Strategies.- Regeneration in Arabidopsis Tissue Culture.- Isolation and Analysis of mRNAs from Specific Cell Types of Plants by Ribosome Immunopurification.- Analyzing Small and Long RNAs in Plant Development Using Non-Radioactive in-situ Hybridization.- Analyzing Protein Distribution in Plant Tissues Using “Whole-Mount” Immunolocalization.- Culture Methods and Mutant Generation in the Filamentous Brown Algae Ectocarpus siliculosus.- Building Simulation Models of Developing Plant Organs Using VirtualLeaf.