Plant Metabolic Flux Analysis, Softcover reprint of the original 1st ed. 2014 Methods and Protocols Methods in Molecular Biology Series, Vol. 1090

Application of Metabolic Flux Analysis to Plants.- Metabolic Network Reconstruction and Their Topological Analysis.- ¹⁴C pulse Labeling to Estimate External Fluxes and Turnovers in Primary Metabolism.- Optimization of Steady-state ¹³C-labeling Experiments for Metabolic Flux Analysis.- Quantification of ¹³C Enrichments and Isotopomer Abundances for Metabolic Flux analysis using 1D NMR Spectroscopy.- Analysis of Proteinogenic Amino Acid and Starch Labeling by 2D NMR.- Analysis of Kinetic Labeling of Amino Acids and Organic Acids by GC-MS.- Quantifying ¹³C-labeling in Free Sugars and Starch by GC-MS.- Liquid Chromatography Tandem Mass Spectrometry for Measuring ¹³C-labeling in Intermediates of the Glycolysis and Pentose-phosphate Pathway.- In Vivo NMR for ¹³C metabolic  Flux Analysis.- Steady State and Instationary Modeling of Proteinogenic and Free Amino Acid Isotopomers for Flux Quantification.- Isotopically Nonstationary MFA (INST-MFA) of Autotrophic Metabolism.- Simulating Labeling to Estimate Kinetic Parameters for Flux Control Analysis.- High-throughput Data Pipelines for Metabolic Flux Analysis in Plants.- Analysis of Enzyme Activities.- Analytical Kinetic Modeling: A Practical Procedure.- Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling.- Flux Variability Analysis: Application to Developing Oilseed Rape Embryos using Toolboxes for Constraint-Based Modeling.- Plant Genome-Scale Modeling and Implementation.- ³⁴S and ¹⁵N Labelling to Model S and N Flux in Plants and Determine the Different Components of N and S use Efficiency.- Ecophysiological Process-based Model to Simulate Carbon Fluxes in Plants.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts