Plant Gravitropism, Softcover reprint of the original 1st ed. 2015 Methods and Protocols Methods in Molecular Biology Series, Vol. 1309

This volume provides the plant scientific community with a collection of established and recently developed experimental protocols to study plant gravitropism. The first few chapters in this book discuss topics such as methods to properly orient plant material for gravitropism studies; protocols for data collection and image analysis; and techniques to investigate ion, organelle, and auxin transporter dynamics, particularly in living cells, as the plant is responding to a change in its orientation. The next few chapters talk about topics that are essential for understanding the complexities underlying tropisms and plant movements in general, and outline basic protocols on handling ornamental flowering shoots for basic plant gravitropism studies. The book concludes with chapters that discuss plant biological studies in space in order to take advantage of unique microgravity conditions not available in Earth-based studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Informative and cutting-edge, Plant Gravitropism is the perfect book for researchers in the plant scientific community because it is not only useful for plant gravitropism studies, but also addresses a range of interesting problems in plant growth and development.

Immunohistochemistry Relative to Gravity: A Simple Method to Retain Information About Gravity for Immunolocalization and Histochemistry.- A Flat Embedding Method to Orient Thin Biological Samples for Sectioning.- Quantification of Root Gravitropic Response Using a Constant Stimulus Feedback System.- The Analysis of Gravitropic Setpoint Angle Control in Arabidopsis.- Imaging of Dynamic Ion Signaling During Root Gravitropism.- Live Cell Imaging of Cytoskeletal and Organelle Dynamics in Gravity-Sensing Cells in Plant Gravitropism.- Auxin Carrier and Signaling Dynamics During Gravitropic Root Growth.- Imaging and Quantitative Methods for Studying Cytoskeletal Rearrangements during Root Development and Gravitropism.- Methods for RNA Profiling of Gravi-Responding Plant Tissues.- Proteomic Approaches and their Application to Plant Gravitropism.- Assays for Root Hydrotropism and Response to Water Stress.- Evaluating Mechano-Transduction and Touch Responses in Plant Roots.- Expressing and Characterizing Mechanosensitive Channels in Xenopus Oocytes.- Flowering Shoots of Ornamental Crops as a Model to Study Cellular and Molecular Aspects of Plant Gravitropism.- Studying Molecular Changes During Gravity Perception and Response in a Single Cell.- Live Cell and Immuno-Labeling Techniques to Study Gravitational Effects on Single Plant Cells.- Use of High Gradient Magnetic Fields to Evaluate Gravity Perception and Response Mechanisms in Plants and Algae.- Use of Microgravity Simulators for Plant Biological Studies.- Conducting Plant Experiments in Space.- Spaceflight Exploration in Plant Gravitational Biology.- Hypergravity Experiments to Evaluate Gravity Resistance Mechanisms in Plants.