Plant Embryogenesis, 2008 Methods in Molecular Biology Series, Vol. 427

Contents PART I. MODEL EMBRYONIC SYSTEMS 1. Arabidopsis embryogenesis Soomin Park and John J. Harada 2. Maize embryogenesis Pilar Fontanet and Carlos M. Vicient 3. Spruce embryogenesis Sara von Arnold and David Clapham PART II. CELLULAR, GENETIC AND MOLECULAR MECHANISMS OF PLANT EMBRYOGENESIS 4. In vitro fertilization with isolated higher plant gametes Erhard Kranz, Yoichiro Hoshino and Takashi Okamoto 5. In vitro culture of Arabidopsis embryos Michael Sauer and Jirí Friml 6. Culture and time-lapse tracking of barley microspore-derived embryos Simone de F. Maraschin, Sandra van Bergen, Marco Vennik and Mei Wang 7. Isolation of embryo-specific mutants in Arabidopsis: Plant transformation Nai-You Liu, Zhi-Feng Zhang and Wei-Cai Yang 8. Isolation of embryo-specific mutants in Arabidopsis: Genetic and phenotypic analysis Nai-You Liu, Zhi-Feng Zhang and Wei-Cai Yang 9. Laser-capture microdissection to study global transcriptional changes during plant embryogenesis Stuart A. Casson, Matthew W.B., Spencer and Keith Lyndsey 10. Promoter trapping system to study embryogenesis Robert Blanvillain and Patrick Gallois 11. Visualization of auxin gradients in embryogenesis Michael Sauer and Jirí Friml 12. Intercellular trafficking of macromolecules during embryogenesis Insoon Kim and Patricia C. Zambryski 13. Immunolocalisation of proteins in somatic embryos: applications for studies on the cytoskeleton Andrei P. Smertenko and Patrick J. Hussey 14. Detection of programmed cell death in plant embryos Lada H. Filonova, Maria F. Suarez and Peter V. Bozhkov