Plant Cytogenetics, Softcover reprint of the original 1st ed. 2016 Methods and Protocols Methods in Molecular Biology Series, Vol. 1429

This volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gene locations, to manipulating and dissecting chromosomes, and then focusing on less understood features of chromosomes such as recombination initiation sites and epigenomic marks. The methods described in Plant Cytogenetics: Methods and Protocols build on each other and provide, those new to the field, with a comprehensive platform to support their research endeavours, while also introducing advanced techniques to experienced researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Plant Cytogenetics: Methods and Protocols, is a valuable resource for anyone who is interested in the diverse and wonderfully complex field of cytogenetics. 

1. C-Banding of Plant Chromosomes
Eric N. Jellen

2. Chromosome Painting by GISH and Multi-Color FISH
Steven S. Xu, Zhao Liu, Qijun Zhang, Zhixia Niu, Chao-Chien Jan, and Xiwen Cai

3. Fluorescent In Situ Hybridization on Extended Chromatin Fibers for High-Resolution Analysis of Plant Chromosomes
Daryna Dechyeva and Thomas Schmidt

4. Tyramide Signal Amplification: FluorescenceIn Situ Hybridization for Identifying Homologous Chromosomes
Araceli Fominaya, Yolanda Loarce, Juan M. González, and Esther Ferrer

5. Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH
Miroslava Karafiátová, Jan Bartoš, Jaroslav Doležel

6. Immunolabelling and In Situ Labelling of Isolated Plant Interphase Nuclei
Ali Pendle and Peter Shaw

7. Manipulation of Homologous Chromosome Recombination in Wheat
Adam J. Lukaszewski

8. Dissecting Plant Chromosomes by the Use of Ionizing Radiation
Penny M.A. Kianian, Katie L. Liberatore, Marisa Miller, Justin B. Hegstad, and Shahryar F. Kianian

9. Optical Nano-Mapping and Analysis of Plant Genomes
Ming-Cheng Luo, Karin R. Deal, Armond Murray, Tingting Zhu, Alex R. Hastie, Will Stedman, Henry Sadowski, and Michael Saghbini

10. Flow Sorting Plant Chromosomes
Jan Vrána, Petr Cápal, Jarmila Číhalíková, Marie Kubaláková, and Jaroslav Doležel

11. Construction of BAC Libraries from Flow-Sorted Chromosomes
Jan Šafář, Hana Šimková, and Jaroslav Doležel

12. The Chromosome Microdissection and Microcloning Technique
Ying-Xin Zhang, Chuan-Liang Deng, and Zan-Min Hu

13. Immunolocalization on Whole Anther Chromosome Spreads for Male Meiosis
Stefanie Dukowic-Schulze, Anthony Harris, and Changbin Chen

14. Mapping Recombination Initiation Sites Using Chromatin Immunoprecipitation
Yan He, Minghui Wang, Qi Sun, and Wojciech P. Pawlowski

15. Chromatin Immunoprecipitation to Study Plant Epigenome
Zidian Xie and Gernot Presting

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras