Permeability Barrier, Softcover reprint of the original 1st ed. 2011 Methods and Protocols Methods in Molecular Biology Series, Vol. 763

The significant biological subject, the permeability barrier, is incredibly diverse and vital for a vast assortment of crucial functions in the body.  In Permeability Barrier: Methods and Protocols, a variety of experienced researchers contribute techniques to study this complex system in its many forms.  Written in the highly successful Methods in Molecular Biology? series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Computational Modeling of the Skin Barrier.- In Vitro Human Skin Segmentation and Drug Concentration-Skin Depth Profiles.- Transcriptional Regulation of Epidermal Barrier Formation.- Epidermal Permeability Barrier  (EPB) Measurement in Mammalian Skin.- Assessment of Permeability Barriers to Macromolecules in the Rodent Endometrium at the Onset of Implantation.- Assessment of Intestinal Permeability in (Premature) Neonates by Sugar Absorption Tests.- Analysis of Epithelial Cell Shedding and Gaps in the Intestinal Epithelium.- Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells.- Optimization of the Caco-2 Permeability Assay to Screen Drug Compounds for Intestinal Absorption and Efflux.- Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion.- Monitoring of the Dynamics of Epithelial Dome Formation Using a Novel Culture Chamber for Long-Term Continuous Live-Cell Imaging.- Measuring Permeability in Human Retinal Epithelial Cells (ARPE-19): Implications for the Study of Diabetic Retinopathy.- Analysis of Epithelial Barrier Integrity in Polarized Lung Epithelial Cells.- Permeability of Differentiated Human Urothelium In Vitro.- Phenotyping the Claudin 11 Deficiency in Testis: From Histology to Immunohistochemistry.- An In Vitro System to Study Sertoli Cell Blood-Testis Barrier Dynamics.- Analysis of Endothelial Barrier Function In Vitro.- Role of Endothelial Cell-Cell Junctions in Endothelial Permeability.- In Vitro Analyses of Endothelial Cell Permeability.- Mechano-Transduction and Barrier Regulation in Lung Microvascular Endothelial Cells.- Role of Caveolin-1 in the Regulation of Pulmonary EndothelialPermeability.- Assessment of Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cell Monolayers.- Permeability of Endothelial Barrier: Cell Culture and In Vivo Models.- Size-Selective and In Vitro Assessment of Inner Blood Retina Barrier Permeability.- Assessment of Permeability in Barrier Type of Endothelium in Brain Using Tracers: Evans Blue, Sodium Fluorescein, and Horseradish Peroxidase.- In Vitro and In Vivo Methods for Assessing FcRn-Mediated Reverse Transcytosis Across the Blood-Brain Barrier.- Evaluation of VEGF-Induced Vascular Permeability in Mice.- In Vivo Measurement of Glioma-Induced Vascular Permeability.- In Vivo Optical Imaging of Ischemic Blood Brain Barrier Disruption.