PCR Primer Design (3rd Ed., 3rd ed. 2022) Methods in Molecular Biology Series, Vol. 2392
Coordonnateur : Basu Chhandak
This third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer design to identify plant and animal viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, PCR Primer Design, Third Edition aims to be useful for various fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.
SECTION I. Primer design for genotyping
M. Delghandi, M.P. Delghandi, S. Goddard
2. Enhancing cohort PASA efficiency from lessons assimilated by mutant genotyping in C. elegans.
Amita Pandey, Binu Bhat, Madan L. Aggarwal, Girdhar K. Pandey
3. Design of oligonucleotides for allele-specific amplification based on PCR and isothermal techniques
Luis Antonio Tortajada-Genaro4. Detection of rubella virus by tri-primer RT-PCR assay and genotyping by fragment RT-PCR.
Suji George
5. Design of mismatch primers to identify and differentiate closely related (sub)species – application to the authentication of meat products
Maria Kaltenbrunner, Rupert Hochegger, Margit Cichna-Markl
6. Primer design for the analysis of closely related species – application of non-coding mtDNA and cpDNA sequences
Lidia Skuza7. Designing PCR primers for the amplification-refractory mutation system
Majid Komijani, Khashayar Shahin, Esam Ibraheem Azhar, Mohammad Bahram
SECTION II. Primer design for genome-wide identification of specific regions
8. Validation of circular RNAs by PCR
Aniruddha Das, Debojyoti Das, and Amaresh C. Panda
9. Primer Designing for Amplifying an AT-Rich Promoter from Arabidopsis thaliana
Pinky Dhatterwal, Sandhya Mehrotra, and Rajesh Mehrotra
SECTION III. Primer design for multiplex PCR. Multiplex
10. PLASmid TAXonomic PCR (PlasTax-PCR), a multiplex relaxase MOB typing to assort plasmids into taxonomic units
Raquel Cuartas, Teresa M. Coque, Fernando de la Cruz, M. Pilar Garcillán-Barcia
11. Multiplex PCR Design for Scalable Resequencing
Darren Korbie, Matt Trau
SECTION IV. Primer design for qPCR
12. Identification of gene copy number in the transgenic plants by quantitative polymerase chain reaction (qPCR)
Poonam Kanwar, Soma Ghosh, Sibaji K. Sanyal, Girdhar K. Pandey
13. qPrimerDB: A powerful and user-friendly database for qPCR primer design
Wei Chang, Yue Niu, Mengna Yu, Tian Li, Jiana Li, Kun Lu
SECTION V. Primer design for identification of plant and animal viruses
14. PCR primer design for the rapidly evolving SARS-CoV-2 genome
Jiangyu Li, Dongsheng Zhao, Haoyang Cai and Wubin Qu
15. Universal primers for detection of novel plant capsid-less viruses: Papaya umbra-like viruses as example
Jorge H. Ramirez-Prado and Luisa A. Lopez-Ochoa
SECTION VI. Use of software for primer design
16. A guide to using FASTPCR software for PCR, in silico PCR, and oligonucleotide analysis
Ruslan Kalendar
SECTION VII. Primer design for newer PCR approaches
17. Pyrosequencing Primer Design for Forensic Biology Applications
Kelly M. Elkins
18. Phosphate methylated oligonucleotides as a novel primer for PCR and RT-PCR
Yu-Hsuan Chang, Meng-Wei Wu, Yi-Ju Chen, Cao-An Vu, Ching-Ya Hong, Wen-Yih Chen
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible result
Contains key notes and implementation advice from the experts
Date de parution : 11-2022
Ouvrage de 276 p.
17.8x25.4 cm
Date de parution : 11-2021
Ouvrage de 276 p.
17.8x25.4 cm
