Nucleosomes, Histones and Chromatin Part A
Auteur : WU Carl
1. Methods applied to the study of Protein Arginine Methylation
2. TBC
3. Genome-wide mapping of nucleosomes in budding yeast
4. High resolution identification of intra- and interchromosomal DNA interactions by 4C technology
5. TBC
6. High throughput assays to identify small-molecule inhibitors of chromatin 'reader' proteins
7. Peptide microarrays to interrogate the ‘histone code’
8. Preparation of Drosophila cells from different stages of the cell cycle for ChIP using centrifugal elutriation and FACS
9. Analysis of Single and Combinatorial Histone PTM Patterns by High Resolution Mass Spectrometry
10. Measuring nucleosome turnover dynamics genome-wide
11. Comparative mRNA expression-profiling of chromatin factors
12. High-Throughput MAPit Single-Molecule Footprinting
13. Genome-wide in vitro reconstitution
14. Quantitating interactions of nuclear factors with chromatin
15. Biochemical assay for histone H2AZ (Htz1) replacement by the yeast SWR1 chromatin remodeling complex
16. TBC
17. TBC
- Continues the legacy of this premier serial by containing quality chapters authored by leaders in the field
- The first of 2 volumes covering nucleosomes, histones and chromatin
- Chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins
Date de parution : 10-2012
Ouvrage de 390 p.
15x22.8 cm
Mots-clés :
5-Carboxylcytosine; 5-Formylcytosine; 5-Hydroxymethylcytosine; Affinity; AlphaScreen; Aminoethylcysteine; Antisilencing function 1; Binding affinity; Binding biophysics; CARM1; Cell cycle; Chromatin; Chromatin remodeling; Cysteine alkylation; DNA methylation; DNA uncoiling; Effector; Electrophoretic mobility shift assay; Epigenetic; Epigenetic inheritance; Epigenetics; Fluorescence; FRET; H2A.Z; H2B-H2A; H3-H4; High-performance liquid chromatography; High-throughput screen; Histone code; Histone exchange; Histone H3/H4; Histone methylation; Histone modification; Histone modifications; Histone octamer; Histone posttranslational modifications; Histone variant; Histones; Htz1; Interaction; Isw1; ITC; Mass spectrometry; Methyl-lysine analogue; MLA; Nap1; Nucleosome; Nucleosome assembly; Oxidation; Peptide microarray; Peptide pull-downs; Peptide synthesis; PHD finger; Posttranslational modifications; PRMT1; PRMT5; Propionylation; Quantitative; Quantitative mass spectrometry; SILAC; Small molecule; Stable isotope labeling; Stoichiometry; Structures; Swr1; Tandem tudor domain; Tet proteins; Tetrasome; TLC; Transcript elongation
