Mouse Cell Culture, Softcover reprint of the original 1st ed. 2010 Methods and Protocols Methods in Molecular Biology Series, Vol. 633

The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies.- Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures.- Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells.- The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference.- Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium, or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation.- Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine.- Isolation and Culture of Embryonic Pancreas and Liver.- Isolation and Culture of Mouse Satellite Cells.- Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures.- Short- and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes.- Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells.- Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes.- Isolation and Culture of Adult Mouse Hepatocytes.- Isolation and Culture of Mouse Intestinal Cells.- Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse.- The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry.- Cell Culture of Primary Cerebellar Granule Cells.- Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain.

Features methods involving a wide variety of tissue types in one convenient volume Written by leading experts in an easy-to-use step-by-step format with notes providing practical, straight-from-the-lab knowledge and experiences Covers protocols with fetal tissues and stem cells as well as cultures of isolated cell types for the study of developmental processes and homogeneous cell populations, respectively Includes supplementary material: sn.pub/extras