Molecular Profiling (2^nd Ed., Softcover reprint of the original 2nd ed. 2017) Methods and Protocols Methods in Molecular Biology Series, Vol. 1606

This second edition provides an update on technology and an accelerated tutorial to assist, students, entrepreneurs, new investigators, and established investigators who want to quickly become versed in, and immersed in, the entire process from discovery to clinical trial validation and commercial public benefit.  Chapters aim to cover the full spectrum of molecular profiling from tumor staging and grading through biomarker discovery, to commercialization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Profiling: Methods and Protocols, Second Edition aims to be a useful guide on molecular profiling. 

1.  Tumor Staging and Grading: A Primer

Stacy M. Telloni

2. Innovations in Clinical Trial Design in the Era of Molecular Profiling

Julia D. Wulfkuhle, Alexander Spira, Kirsten H. Edmiston, and Emanuel F. Petricoin

3. Personalized Medicine: Ethical Aspects

Michele Signore, Valeria Manganelli and Alex Hodge

5. Scanning Electron Microscopy Sample Preparation and Imaging

Jenny Ngoc Tran Nguyen and Amanda M. Harbison

Claudius Mueller, Michael G. Harpole, and Virginia Espina

Ruben Magni and Alessandra Luchini

Brian A. Corgiatand Claudius Mueller

Brianna Kim

Justin B. Davis

11. Reverse Phase Protein Microarrays

Elisa Baldelli, Valerie Calvert, Alex Hodge, Amy VanMeter, Emanuel F. Petricoin III, and Mariaelena Pierobon

12. Clustering and Network Analysis of Reverse-Phase Protein Array Data

Adam Byron

13. PCR: Identification of Genetic Polymorphisms

Amanda M. Harbison and Jenny Ngoc Tran Nguyen

14. Microsatellite Analysis for Identification of Individuals Using Bone from the Extinct Steller’s Sea Cow (Hydrodamalis gigas)

Jeffery F. Warner, Michael G. Harpole, and Lorelei D. Crerar < 

15. Somatic DNA Mutation Analysis

Anthony O’Grady and Robert Cummins

16. Optimization of Immunostaining for Prospective Image Analysis

Scott M. Lawrence and Yelena G. Golubeva

17. Fluorescence in situ Hybridization of Cells, Chromosomes, and Formalin Fixed Paraffin Embedded Tissues

Ahmad Alamri, Jun Yeb Nam, and Jan K. Blancato

18. High-Resolution Image Stitching as a Tool to Assess Tissue-Level Protein Distribution and Localization

Bryan A. Millis and Matthew J. Tyska

19. Mass Spectrometry Based Biomarker Discovery

Weidong Zhou, Emanuel F. Petricoin III, and Caterina Longo

20. Quantitative Mass Spectrometry by Isotope-Dilution and Multiple Reaction Monitoring (MRM)

 Paul Russo, Brian L. Hood, Nicholas W. Bateman, Thomas P. Conrads

22. Metabolomic Bioinformatic Analysis

23. Stable Isotope Quantitative N-glycan Analysis by Liquid Separation Techniques and Mass Spectrometry

Stefan Mittermayr, Simone Albrecht, Csaba Váradi, Silvia Millán-Martín,and Jonathan Bones

24. Grant Writing Tips for Translational Research

Lindsay Wescott, Michael Laskofski, Donna Senator, and Carly Curran

Hina Mehta, Lille Tidwell, and Lance A. Liotta

26. Product Development and Commercialization of Diagnostic or Life Science Products for Scientists and Researchers

Meghan M. Alonso

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras