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Molecular diversity and pcr-detection of toxigenic fusarium species and ochratoxigenic fungi (reprinted from european journal of plant

Langue : Anglais

Auteurs :

Toxigenic Fusarium species and ochratoxigenic fungi are responsible for plant diseases which have important effects on both human and animal health. The development of rapid, robust and sensitive detection methods based on molecular technologies is needed to identify fungal contamination in the field and quantify toxin accumulation in food products. Most of the contributions are from results obtained through the EU 5th Framework project (QLK1-1999-01380) "DETOX-FUNGI: early detection of toxigenic Fusarium species and ochratoxigenic fungi in plant products". Valuable contributions from other scientists have guaranteed a complete overview of increasingly important topic.
The present issue contains contributions which cover many aspects of the molecular diversity, phylogeny, variability and PCR-detection of toxigenic fungi and their toxins. Notable amongst these are a review of the phylogeny and diagnosis of Fusarium species and a new system for identifying Fusarium species based on a DNA sequence database. Other papers describe the diversity and analysis of ochratoxigenic Aspergilli and Penicillia. This information will greatly aid those concerned with the early detection of these fungi and their mycotoxins, information which is so essential for overcoming the health risks associated with mycotoxin-contaminated food products.
  • Phylogeny and molecular diagnosis of mycotoxigenic fungi, K.A. Seifert, C.A. Lévesque.
  • FUSARIUM-ID v 1.0: a DNA sequence database for identifying Fusarium, D.M. Geiser, et al.
  • Quantitative detection of Fusarium species in wheat using TaqMan, C. Waalwijk, et al.
  • A species-specific PCR assay based on the calmodulin partial gene for identification of Fusarium verticillioides, F. Profileratum and F. Subglutinans, G. Mulè.
  • Detection and differentiation of trichothecene and enniatin-producing Fusarium species on small-grain cereals, P. Nicholson, et al.
  • Differentiation of Fusarium verticillioides from banana fruits by IGS and EF-1 sequence analyses, S. Mirete, et al.
  • Genetic markers for the analysis of variability and for production of specific diagnostic sequences in fumonisin-producing strains of Fusarium verticillioides, M.T. González-Jaén, et al.
  • Synteny in toxigenic Fusarium species: the fumonisin gene cluster and the mating type region as examples, C. Waalwijk, et al.
  • Mitochondrial DNA diversity and lineage determination of European isolates of Fusarium graminearum (Gibberella zeae), M. Láday, et al.
  • Genetic diversity of Fusarium graminearum in Europe and Asia, T.Y. Gagkaeva, T. Yli-Mattila.
  • Mitochondrial DNA variability in Fusarium proliferatum (Gibberella intermedia), M. Láday, et al.
  • Molecular and morphological diversity of Fusarium species in Finland and north western Russia, T. Yli-Mattila, et al.
  • Mycotoxin production and molecular variability of European and American isolates of Fusarium culmorum, B. Tó,th, et al.
  • Toxin profile, fertility and AFLP analysis of Fusarium verticillioides from banana fruits, A. Moretti, et al.
  • Infertility of two mating populations in the Gibberella fujikuroi species complex, J.F. Leslie, et al.
  • Identification of growth stage specific transcripts profiles in Fusarium proliferatum (Gibberella fujikuroi mating population D) by cDNA-AFLP analysis, A. Jeney, et al.
  • Molecular diversity of agriculturally important Aspergillus species, J. Varga, et al.
  • PCR assay for identification of Aspergillus carborarius and Aspergillus japonicus, G. Perrone, et al.
  • Production of ochratoxin A by Aspergillus ochraceus, E. Mü,hlencoert, et al.
  • Analysis of differentially-expressed ochratoxin A biosynthesis genes of Penicillium nordicum, P. Fä,rber, R. Geisen.

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