Mitochondrial Medicine, Softcover reprint of the original 1st ed. 2015 Volume I, Probing Mitochondrial Function Methods in Molecular Biology Series, Vol. 1264

This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. Mitochondrial Medicine: Volume I, Probing Mitochondrial Function focuses on methods being used for the assessment of mitochondrial function under physiological conditions as well as in healthy isolated mitochondria. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Mitochondrial Medicine provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.

1. Preparation of ‘Functional’ Mitochondria: A Challenging Business

            Stefan Lehr, Sonja Hartwig, and Jorg Kotzka

2. Isolation and Quality Control of Functional Mitochondria

            Sonja Hartwig, Jorg Kotzka, and Stefan Lehr

3. Isolation of Mitochondria-Associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs) from Brain Tissues and Neuronal Cells

            Ida Annunziata, Annette Patterson, and Alessandra d’Azzo

4. Fluorescence Analysis of Single Mitochondria with Nanofluidic Channels

            Ted Pham, Katayoun Zand, Douglas Wallace, and Peter Burke

5. Optical Microwell Arrays for Large Scale Studies of Single Mitochondria Metabolic Responses

            Venkata Suresh Vajrala, Emmanuel Suraniti, Bertrand Goudeau, Neso Sojic, and Stéphane Arbault

6. Deep Resequencing of Mitochondrial DNA

            Brendan A.I. Payne, Kristian Gardner, Jonathan Coxhead, and Patrick F. Chinnery

7. Single Cell Analysis of Mitochondrial DNA

            Brendan A.I. Payne,  Lynsey Cree, and Patrick F. Chinnery

8. A High-Throughput Next-Generation Sequencing Assay for the Mitochondrial Genome

            Shale Dames, Karen Eilbeck, and Rong Mao

9. Rapid Mitochondrial DNA Isolation Method for Direct Sequencing

            Wilber Quispe-Tintaya, Ryan R. White, Vasily N. Popov, Jan Vijg, and Alexander Y. Maslov

10. Analysis of Mitochondrial DNA and RNA Integrity by a Real-Time qPCR Based Method

            Wei Wang, Ying Esbensen, Katja Scheffler, and Lars Eide

11. Mitochondria-Targeted RNA Import

            Geng Wang, Eriko Shimada, Mahta Nili, Carla M. Koehler, and Michael A. Teitell

12. Accurate Measurement of Circulating Mitochondrial DNA Content from Human Blood Samples Using Real Time Quantitative PCR

            Saima Ajaz, Anna Czajka, and Afshan Malik

13. mTRIP: An Imaging Tool to Investigate Mitochondrial DNA Dynamics in Physiology and Disease at the Single-Cell Resolution

            Laurent Chatre and Miria Ricchetti

14. Simultaneous Quantification of Mitochondrial ATP and ROS Production

            Liping Yu, Brian D. Fink, and William I. Sivitz

15. Live-Cell Assessment of Mitochondrial Reactive Oxygen Species Using Dihydroethidine

            Marleen Forkink, Peter H.G.M. Willems, Werner J.H. Koopman, and Sander Grefte

16. Detection and Differentiation Between Peroxynitrite and Hydroperoxides Using Mitochondria-Targeted Arylboronic Acid

            Jacek Zielonka, Adam Sikora, Jan Adamus, and B. Kalyanaraman

17. Time-Resolved Spectrometry of Mitochondrial NAD(P)H Fluorescence and Its Applications for Evaluating the Oxidative State in Living Cells

            Julia Horilova, Hauke Studier, Zuzana Nadova, Pavol Miskovsky, Dusan Chorvat, Jr., and Alzbeta Marcek Chorvatova

18. Novel Methods for Measuring the Mitochondrial Membrane Potential

            Roger Springett

19. High-Throughput Real-Time Analysis of Cell Oxygenation Using Intracellular Oxygen-Sensitive Probes

            James Hynes and Conn Carey

20. In Vivo Assessment of Mitochondrial Oxygen Consumption

            Floor A. Harms and Egbert G. Mik

21. Imaging Mitochondrial Hydrogen Peroxide in Living Cells

            Alexander R. Lippert, Bryan C. Dickinson, and Elizabeth J. New

22. Simultaneous High-Resolution Measurement of Mitochondrial Respiration and Hydrogen Peroxide Production

            Gerhard Krumschnabel, Mona-Fontana-Ayoub, Zuzana Sumbalova, Juliana Heidler, Kathrin Gauper, Mario Fasching, and Erich Gnaiger

23. Measurement of Mitochondrial NADH and FAD Autofluorescence in Live Cells

            Fernando Bartolomé and Andrey Y. Abramov

24. Mitochondrial Coenzyme Q10 Determination Via Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry

            Outi Itkonen and Ursula Turpeinen

25. Assessing the Bioenergetic Profile of Human Pluripotent Stem Cells

            Vanessa Pfiffer and Alessandro Prigione

26. Integrative Methods for Studying Cardiac Energetics

            Philippe Diolez, Véronique Deschodt-Arsac, Guillaume Calmettes, Gilles Gouspillou, Laurent Arsac, Pierre dos Santos, Pierre Jais, and Michel Haissaguerre

27. Computer-Based Prediction of Mitochondria-Targeting Peptides

            Pier Luigi Martelli, Castrense Savojardo, Piero Fariselli, Gianluca Tasco, and Rita Casadio

28. Prediction of Mitochondrial Protein Function by Comparative Physiology and Phylogenetic Profiling

            Yiming Cheng and Fabiana Perocchi

29. Assessment of Posttranslational Modification of Mitochondrial Proteins

            Sudharsana R. Ande, G. Pauline Padilla-Meier, and Suresh Mishra

30. Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway

            Ana S. Almeida and Helena L.A. Vieira

31. High-Resolution Melting Analysis for Identifying Sequence Variations in Nuclear Genes for Assembly Factors and Structural Subunits of Cytochrome C Oxidase

            Alžběta Vondráčková, Kateřina Veselá, Jiří Zeman, and Markéta Tesařová

32. Heterologous Inferential Analysis (HIA) as a Method to Understand the Role of Mitochondrial rRNA Mutations in Pathogenesis

            Joanna L. Elson, Paul M. Smith, and Antón Vila-Sanjurjo

33. Analysis of Mitochondrial Dysfunction During Cell Death

            Vladimir Gogvadze, Sten Orrenius, and Boris Zhivotovsky

34. The Use of FLIM-FRET for the Detection of Mitochondria-Associated Protein Interactions

            Elizabeth J. Osterlund, Qian Liu, and David W. Andrews

35. Assessment of Mitochondrial Ca²⁺ Uptake

            András T. Deak, Claire Jean-Quartier, Alexander I. Bondarenko, Lukas N. Groschner, Roland Malli, Wolfgang F. Graier, and Markus Waldeck-Weiermair

36. Qualitative Characterization of the Rat Liver Mitochondrial Lipidome Using All Ion Fragmentation on an Exactive Benchtop Orbitrap MS

            Susan S. Bird, Irina G. Stavrovskaya, Rose M. Gathungu, Fateme Tousi, and Bruce S. Kristal

37. Characterization of Mitochondrial Populations During Stem Cell Differentiation

            Petra Kerscher, Blakely S. Bussie, Katherine M. DeSimone, David A. Dunn, and Elizabeth A. Lipke

38. An Ex Vivo Model for Studying Mitochondrial Trafficking in Neurons

            Helena Bros, Raluca Niesner, and Carmen Infante-Duarte

Includes cutting-edge methods and protocols for the assessment of mitochondrial function

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts