Microfluidic Diagnostics, Softcover reprint of the original 1st ed. 2013 Methods and Protocols Methods in Molecular Biology Series, Vol. 949

Microfluidic techniques are becoming widely incorporated into medical diagnostic systems due to the inherent advantages of miniaturization. In Microfluidic Diagnostics: Methods in Molecular Biology, researchers in the field detail methods and protocols covering subjects such as microfluidic device fabrication, on-chip sample preparation, diagnostic applications and detection methodologies. The protocols described range from cutting-edge developments to established techniques and basic demonstrations suitable for education and training; from basic fabrication methods to commercializing research. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Present Technology and Future Trends in Point of Care Microfluidic Diagnostics.-Teaching Microfluidic Diagnostics Using Jell-O^® Chips.-Fundamentals of Microfluidics for High School Students with no Prior Knowledge of Fluid Mechanics.-Measuring Microchannel Electroosmotic Mobility and Zeta Potential by the Current Monitoring Method.-Overview of the Microfluidic Diagnostics Commercial Landscape.-Practical Aspects of the Preparation and Filing of Patent Applications.-Introduction to In Vitro Diagnostic Device Regulatory Requirements.-Microfluidic Device Fabrication by Thermoplastic Hot-Embossing.-Introduction to Glass Microstructuring Techniques.-Glass Microstructure Capping and Bonding Techniques.-Rapid Prototyping of PDMS Devices using SU‑8 Lithography.-Microfluidic Interface Technology based on Stereolithography for Glass-based Lab-on-a-Chips.-Three-Dimensional, Paper-Based Microfluidic Devices Containing Internal Timers for Running Time-Based Diagnostic Assays.-Thread Based Devices for Low-Cost Diagnostics.-Droplet Based Microfluidics.-Droplet-Based Microfluidics for Binding Assays and Kinetics Based on FRET.-Surface Treatments for Microfluidic Biocompatibility.-Superhydrophobicity for Anti-Fouling Microfluidic Surfaces.-The Application of Microfluidic Devices for Viral Diagnosis in Developing Countries.-Applications of Microfluidics for Molecular Diagnostics.-Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers.-Breast Cancer Diagnostics using Microfluidic Multiplexed Immunohistochemistry.-Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis.-Multilayer Microfluidic Poly(ethylene glycol)Diacrylate Hydrogels.-Purification of DNA/RNA in a Microfluidic Device.-Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR.-Integrated Fluidic Circuits (IFCs) for Digital PCR.-microFIND^® Approach to Fluorescent in Situ Hybridization (FISH).-An ELISA Lab-on-a-Chip (ELISA-LOC).-Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis.-Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement.-Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins.