Methods in Membrane Lipids (2^nd Ed., 2nd ed. 2015) Methods in Molecular Biology Series, Vol. 1232

This volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and in silico. Chapters focus on properties of the component lipids, membranes and their biophysical properties, fluorescent probes for studying membranes, sample preparation, physical techniques to study membrane composition, properties , and function, behavior of cholesterol within a bilayer and examination of cholesterol-dependent phase separation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Methods in Membrane Lipids, Second Edition seeks to aid scientist in further study into membrane lipids.

1. Introduction to Membrane Lipids

Richard M. Epand

2. Introduction: Membrane properties (Good) for Life

Marek Cebecauer

3. Introduction To Fluorescence Probing Of Biological Membranes

Alexander P. Demchenko, Guy Duportail, Sule Oncul, Andrey S. Klymchenko, and Yves Mély

4. The Assembly and Use of Tethered Bilayer Lipid Membranes (tBLMs)

Charles Cranfield, Sonia Carne, Boris Martinac,  and Bruce Cornell

5. Preparation of Detergent-Resistant Membranes (DRMs) from Cultured Mammalian Cells

Deborah A. Brown

6. Isolation of Giant Plasma Membrane Vesicles for Evaluation of Plasma Membrane Structure and Protein Partitioning

KR Leventaland  I Levental

7. Asymmetric Giant Lipid Vesicle Fabrication

Peichi C. Hu and Noah Malmstadt

8. Cholesterol Depletion using Methyl-b-cyclodextrin

Saleemulla Mahammad and Ingela Parmryd

9. A Comparative LC-MS Based Profiling Approach to Analyze Lipid Composition in Tissue Culture Systems

G. Ekin Atilla-Gokcumen and Ulrike S. Eggert

10. Imaging Membrane Order using Environmentally Sensitive Fluorophores

G Ashdown and DM Owen

11. 3D Super-resolution Imaging by Localisation Microscopy

Astrid Magenau and Katharina Gaus

12. Electron Microscopy Methods for Studying Plasma Membranes

Alison J. Beckett and Ian A. Prior

13. Measuring Cytoskeleton and Cellular Membrane Mechanical Properties by Atomic Force Microscopy

Charles Roduit, Giovanni Longo, Giovanni Dietler, and Sandor Kasas

14. Fluorescence Linear Dichroism Imaging for Quantifying Membrane Order

Richard KP Benninger

15. Scanning Fluorescence Correlation Spectroscopy on Biomembranes

Eduard Hermann, Jonas Ries, and Ana J. García-Sáez

16. X-ray Diffraction of Lipid Model Membranes

Arwen I.I. Tyler, Robert V. Law, and John M. Seddon

17. Solid State NMR of Lipid Model Membranes

Arwen I.I. Tyler, James A. Clarke, John M. Seddon, and Robert V. Law

18. Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis and Applications

Michael Carnell, Alex Macmillan, and  Renee Whan

19. The Laurdan Spectral Phasor Method To Explore Membrane Micro-Heterogeneity And Lipid Domains In Live Cells

Ottavia Golfetto, Elizabeth Hinde, and Enrico Gratton

20. Cholesterol Behavior in Asymmetric Lipid Bilayers: Insights from Molecular Dynamics Simulations

Semen O. Yesylevskyy and Alexander P. Demchenko

21. Computer Simulations Of Phase Separation In Lipid Bilayers and Monolayers

Svetlana Baoukina and D. Peter Tieleman                                                                                                                                                          

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras