Methods for Analysis of Golgi Complex Function

This new volume of Methods in Cell Biology looks at methods for analyzing of golgi complex function. Chapters cover such topics as in vitro reconstitution systems, fluorescence-based analysis of trafficking in mammalian cells and high content screening. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.

Model systems and functional studies1.Analysis of Golgi Complex Functions: In vitro Reconstitution Systems2.RNA Interference Approaches to Examine Golgi Function in Animal Cell Culture3.Trafficking along the Secretory Pathway in Drosophila Cell Line and Tissues: A Light and Electron Microscopy Approach4.Studying Membrane Trafficking in the Worm C. elegans by RNA Interference5.Endomembrane and Golgi Traffic in Plant Cells6.Biochemical Analysis of Secretory Trafficking in Mammalian Cells 7.Methods for Analysis of Apical Lumen Trafficking Using Micropatterned 3D Systems8.Using Replication Defective Viruses to Analyze Membrane Trafficking in Polarized Epithelial Cells9.Snap-Tagging the Retrograde Route10.Assessing ER and Golgi N-Glycosylation Process using Metabolic Labeling in Mammalian Cultured Cells

Imaging based approaches11.Fluorescence based Analysis of Trafficking in Mammalian Cells12.Photobleaching Methods to study Golgi Complex Dynamics in Living Cells13.Photohighlighting approaches to access membrane dynamics of the Golgi apparatus14.Live Cell Visualization of Golgi Membrane Dynamics by Super-Resolution Confocal Live Imaging Microscopy (SCLIM)15.Analysis of Golgi Complex Function using Correlative Light-Electron Microscopy (CLEM)16.Viewing Golgi Structure and Function from a different perspective – Insights from Electron Tomography17.High Content Screening and Analysis of the Golgi Complex

Emerging studies [New Frontiers]18.(Re)Modeling the Golgi19.Golgi Depletion from Living Cells with Laser Nanosurgery20.Studying Intracellular Trafficking Pathways with Probabilistic Density Maps21.Methods to Study Signaling at The Golgi Apparatus22.Golgi-Dependent Signaling: Self-Coordination of Membrane Trafficking23.Signaling at the Golgi during Mitosis24.Ice Recovery Assay for Detection of Golgi-Derived Microtubules

Researchers and students in cell, molecular and developmental biology

• Covers sections on model systems and functional studies, imaging-based approaches and emerging studies
• Chapters are written by experts in the field
• Cutting-edge material